HEFX1

General#

Cell Line

hPSCreg Name HUJIe001-A
Alternative name(s)
HEFX1
Cell line type Human embryonic stem cell (hESC)
Last update 27th October 2017
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Hebrew University of Jerusalem (HUJI)
Derivation country Israel

External Databases

Cellosaurus CVCL_9837

General Information

Publications
Is the cell line available in principle?
No

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Fragile X syndrome
The donor is affected.
Synonyms
  • FXS
  • FraX syndrome
  • Martin-Bell syndrome
  • FRAXA syndrome
show more synonyms
Disease associated phenotypes
  • Fragile-X Syndrome, carrier (> 500 repeats)

Ethics#

We are currently revising this section.

hESC Derivation#

Embryo stage Blastula with ICM and Trophoblast
PGD Embryo?
Yes
ZP removal technique Mechanical
Cell isolation Immunosurgery
Cell seeding Isolated ICM

Culture Conditions#

Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
ZFP42 (REX-1)
Yes
Alkaline Phosphatase
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY

Other Genotyping (Cell Line)