INSRMe016-A

STR-I-347-FRAXA

General

Cell Line

hPSCreg name INSRMe016-A
Cite as:
INSRMe016-A (RRID:CVCL_C096)
Alternative name(s)
STR-I-347-FRAXA
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 4th September 2022
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Provider

Generator INSERM (INSRM)
Derivation country France

External Databases

Cellosaurus CVCL_C096
Wikidata Q54970630

General Information

* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is affected.
Synonyms
  • FXS
  • FraX syndrome
  • Martin-Bell syndrome
  • FRAXA syndrome
show more synonyms

Ethics

Was the embryo established purely for research purposes? No
Have both parents consented to the use of the embryo for ESC derivation? Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
How may genetic information associated with the cell line be accessed?
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved?
Approval number

hESC Derivation

Embryo stage Blastula with ICM and Trophoblast
PGD Embryo?
Yes
ZP removal technique Enzymatic

Culture Conditions

Surface coating Gelatin
Passage method Mechanically
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine
bFGF

Characterisation

Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)