VUB14

The cell line is not validated yet.

General

Cell Line

hPSCreg Name
VUBe014-A
Alternative name(s)
VUB14
Cell line type Human embryonic stem cell (hESC)
Last update 7th April 2022
User feedback
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Provider

Generator Vrije Universiteit Brussel (VUB)
Derivation country Belgium

External Databases

CLO CLO_0102589
Cellosaurus CVCL_9850
Wikidata Q54993400

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Ethics

Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number
How may genetic information associated with the cell line be accessed?
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Federal Commission Medical and Scientific Research on embryos in vitro (Belgium)
Approval number

hESC Derivation

Date of derivation 2007-02-26

Culture Conditions

Feeder cells Mouse Embryonic Fibroblast Cell
Cellfinder Ont Id: EFO_0004040
O2 Concentration 20 %
CO2 Concentration 10 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine
bFGF %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60
Yes
TRA 1-81
Yes
GDF3
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
ZFP42 (REX-1)
Yes
SOX2
Yes
SSEA-3
Yes
SSEA-4
Yes
GABRB3
Yes
TDGF1
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)