General#

Cell Line
hPSCreg Name A standardised name that was automatically generated based on the cell line information. see Naming Tool	VUBe014-A
Alternative name(s)	VUB20_CMT1A
Cell line type	Human embryonic stem cell (hESC)
Last update	28th July 2017
User feedback show/hide	Written by on Delete No feedback available yet. Login to share your feedback, experiences or results with the research community.
Provider
Generator	Vrije Universiteit Brussel (VUB) Contact: Department of Embryology and Genetics
Distributors	European Collection of Authenticated Cell Cultures (ECACC)
Derivation country	Belgium
External Databases
Cellosaurus	CVCL_9855
CLO	CLO_0102589
Wikidata	Q54993406
General Information
Publications	Mateizel I et al. Derivation, culture, and characterization of VUB hESC lines. In vitro cellular & developmental biology. Animal. 2010 Apr;46(3-4):300-8.
* Is the cell line readily obtainable for third parties?	Yes

Donor Information#

General Donor Information
Sex	female
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Charcot-Marie-Tooth disease type 1 Charcot-Marie-Tooth disease type 1 in the OLS The donor is affected. Synonyms hereditary motor and sensory neuropathy type 1

Ethics#

Alternatives to consent are available?	Yes
Alternatives to consent
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions?	Yes
Name of accrediting authority involved?	Federal Commission Medical and Scientific Research on embryos in vitro (Belgium)

hESC Derivation#

Date of derivation	2007-07-25
Embryo stage	Blastula with ICM and Trophoblast
PGD Embryo?	Yes
ZP removal technique	Spontaneous Hatching
Cell isolation	Mechanical
Cell seeding	Isolated ICM

Culture Conditions#

Feeder cells	Mouse Embryonic Fibroblast Cell Cellfinder Ont Id: EFO_0004040
O2 Concentration	20 %
CO2 Concentration	10 %
Medium	Other medium: Base medium: Knockout-DMEM Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements Supplement Amount Unit L-Glutamine

Characterisation#

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	FACS	Enzymatic Assay	Expression Profiles
Alkaline Phosphatase	Yes
TRA 1-60	Yes
TRA 1-81	Yes
DNMT3B	Yes
GDF3	Yes
NANOG	Yes
POU5F1 (OCT-4)	Yes
ZFP42 (REX-1)	Yes
SOX2	Yes

Genotyping#

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46XX Passage number: 18
Other Genotyping (Cell Line)