VUB20_CMT1A
VUBe014-A
General#
Cell Line |
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hPSCreg Name | VUBe014-A |
Alternative name(s) |
VUB20_CMT1A
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Cell line type | Human embryonic stem cell (hESC) |
Last update | 28th July 2017 |
User feedback | |
Provider |
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Generator | Vrije Universiteit Brussel (VUB) |
Derivation country | Belgium |
External Databases |
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Cellosaurus | CVCL_9855 |
CLO | CLO_0102589 |
General Information |
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Publications | |
* Is the cell line readily obtainable for third parties? |
Yes |
Donor Information#
General Donor Information |
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Sex | female |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
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Ethics#
Alternatives to consent are available? | Yes |
Alternatives to consent | |
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
Name of accrediting authority involved? | Federal Commission Medical and Scientific Research on embryos in vitro (Belgium) |
hESC Derivation#
Date of derivation | 2007-07-25 |
Embryo stage | Blastula with ICM and Trophoblast |
PGD Embryo? |
Yes |
ZP removal technique | Spontaneous Hatching |
Cell isolation | Mechanical |
Cell seeding | Isolated ICM |
Culture Conditions#
Feeder cells |
Mouse Embryonic Fibroblast Cell Cellfinder Ont Id: EFO_0004040 |
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O2 Concentration | 20 % | ||||||
CO2 Concentration | 10 % | ||||||
Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
Alkaline Phosphatase |
Yes |
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TRA 1-60 |
Yes |
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TRA 1-81 |
Yes |
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DNMT3B |
Yes |
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GDF3 |
Yes |
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NANOG |
Yes |
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POU5F1 (OCT-4) |
Yes |
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ZFP42 (REX-1) |
Yes |
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SOX2 |
Yes |
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Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46XX
Passage number: 18
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Other Genotyping (Cell Line) |
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