VUB20_CMT1A
VUBe014-A
General#
Cell Line |
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| hPSCreg Name | VUBe014-A |
| Alternative name(s) |
VUB20_CMT1A
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| Cell line type | Human embryonic stem cell (hESC) |
| Last update | 28th July 2017 |
| User feedback | |
Provider |
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| Generator | Vrije Universiteit Brussel (VUB) |
| Derivation country | Belgium |
External Databases |
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| Cellosaurus | CVCL_9855 |
| CLO | CLO_0102589 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes |
Donor Information#
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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Ethics#
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Federal Commission Medical and Scientific Research on embryos in vitro (Belgium) |
hESC Derivation#
| Date of derivation | 2007-07-25 |
| Embryo stage | Blastula with ICM and Trophoblast |
| PGD Embryo? |
Yes |
| ZP removal technique | Spontaneous Hatching |
| Cell isolation | Mechanical |
| Cell seeding | Isolated ICM |
Culture Conditions#
| Feeder cells |
Mouse Embryonic Fibroblast Cell Cellfinder Ont Id: EFO_0004040 |
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| O2 Concentration | 20 % | ||||||
| CO2 Concentration | 10 % | ||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| DNMT3B |
Yes |
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| GDF3 |
Yes |
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| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| ZFP42 (REX-1) |
Yes |
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| SOX2 |
Yes |
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Genotyping#
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XX
Passage number: 18
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Other Genotyping (Cell Line) |
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