VUB16
The cell line is not validated yet.
VUBe016-A
General
Cell Line |
|
| hPSCreg name | VUBe016-A |
| Cite as: | VUBe016-A (RRID:CVCL_9852) |
| Alternative name(s) |
VUB16
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 7th April 2022 |
| User feedback | |
Provider |
|
| Generator | Vrije Universiteit Brussel (VUB) |
| Derivation country | Belgium |
External Databases |
|
| Cellosaurus | CVCL_9852 |
| Wikidata | Q54993403 |
General Information |
|
| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes |
Donor Information
General Donor Information |
|
Phenotype and Disease related information (Donor) |
|
| Diseases | A disease was diagnosed.
|
Ethics
| How may genetic information associated with the cell line be accessed? |
hESC Derivation
| Embryo stage | Blastula with ICM and Trophoblast |
| ZP removal technique | Spontaneous Hatching |
| Cell isolation | Mechanical |
| Cell seeding | Isolated ICM |
Culture Conditions
| O2 Concentration | 20 % |
| CO2 Concentration | 10 % |
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
Genotyping
Karyotyping (Cell Line) |
|
Other Genotyping (Cell Line) |
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