VUB16

The cell line is not validated yet.

General

Cell Line

hPSCreg Name
VUBe016-A
Alternative name(s)
VUB16
Cell line type Human embryonic stem cell (hESC)
Last update 7th April 2022
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Provider

Generator Vrije Universiteit Brussel (VUB)
Derivation country Belgium

External Databases

Cellosaurus CVCL_9858
CLO CLO_0102591
Wikidata Q54993410

General Information

* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.

Ethics

How may genetic information associated with the cell line be accessed?

hESC Derivation

Embryo stage Blastula with ICM and Trophoblast
ZP removal technique Spontaneous Hatching
Cell isolation Mechanical
Cell seeding Isolated ICM

Culture Conditions

O2 Concentration 20 %
CO2 Concentration 10 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles

Genotyping

Karyotyping (Cell Line)

Other Genotyping (Cell Line)