General

Cell Line
hPSCreg name	VUBe009-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	VUBe009-A (RRID:CVCL_9846)
Alternative name(s)	VUB09_FSHD
Cell line type	Human embryonic stem cell (hESC)
Similar lines	RCi007-A (FB70c1, RCi170) Donor's gene variants: LRIF1, LRIF1 Donor diseases: Facioscapulohumeral dystrophy RCi009-A (Rci201, FB73c6) Donor's gene variants: LRIF1, LRIF1 Donor diseases: Facioscapulohumeral dystrophy RCi005-A (FB71c4, RCi171) Donor's gene variants: LRIF1, LRIF1 Donor diseases: Facioscapulohumeral dystrophy RCi007-C Donor's gene variants: LRIF1 Donor diseases: Facioscapulohumeral dystrophy RCi006-A (FB69c4, RCi169) Donor's gene variants: LRIF1, LRIF1 Donor diseases: Facioscapulohumeral dystrophy
Last update	15th October 2021
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Provider
Generator	Vrije Universiteit Brussel (VUB) Contact: Department of Embryology and Genetics
Derivation country	Belgium
External Databases
Cellosaurus	CVCL_9846
Wikidata	Q54993395
General Information
Publications	Mateizel I et al. Derivation, culture, and characterization of VUB hESC lines. In vitro cellular & developmental biology. Animal. 2010 Apr;46(3-4):300-8.
* Is the cell line readily obtainable for third parties?	Yes

Donor Information

General Donor Information
Sex	female
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Facioscapulohumeral muscular dystrophy The donor is affected. Synonyms Landouzy Dejerine muscular dystrophy Landouzy-Dejerine muscular dystrophy Muscular dystrophy, Landouzy-Dejerine

Ethics

Alternatives to consent are available?	Yes
Alternatives to consent
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions?	Yes
Name of accrediting authority involved?	Federal Commission Medical and Scientific Research on embryos in vitro (Belgium)

hESC Derivation

Date of derivation	2006-04-13
Embryo stage	Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?	No Other hESC source: IVF
PGD Embryo?	Yes
Cell isolation	Immunosurgery
Cell seeding	Isolated ICM

Culture Conditions

Surface coating	MEF
Feeder cells	mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040
O2 Concentration	20 %
CO2 Concentration	10 %
Medium	Other medium: Base medium: Knockout-DMEM Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements Supplement Amount Unit L-Glutamine bFGF

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
Alkaline Phosphatase	Yes
SSEA-1	No
SSEA-3	Yes
SSEA-4	Yes
TRA 1-60	Yes
TRA 1-81	Yes
DNMT3B	Yes
GDF3	Yes
LIN28	Yes
NANOG	Yes
NPM1	Yes
POU5F1 (OCT-4)	Yes
ZFP42 (REX-1)	Yes
SOX2	Yes

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46XX Passage number: 40
Other Genotyping (Cell Line)