VUB19_DM1
The cell line is not validated yet.
VUBe019-A
General
Cell Line |
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| hPSCreg name | VUBe019-A |
| Cite as: | VUBe019-A (RRID:CVCL_9854) |
| Alternative name(s) |
VUB19_DM1
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| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
CENSOi008-A (FB77R2c3, CENSOi260) Donor's gene variants: DMPK, DMPK Donor diseases: myotonic dystrophy type 1 CBRCULi015-A (GM23375-1, GM23375 clone 1) Donor's gene variants: DMPK – DM1 protein kinase, DMPK – DM1 protein kinase Donor diseases: Congenital Myotonic Dystrophy |
| Last update | 27th April 2022 |
| User feedback | |
Provider |
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| Generator | Vrije Universiteit Brussel (VUB) |
| Derivation country | Belgium |
External Databases |
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| Cellosaurus | CVCL_9854 |
| Wikidata | Q54993405 |
General Information |
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| Publications |
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| * Is the cell line readily obtainable for third parties? |
Yes |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Alternative consent approval number | |
| How may genetic information associated with the cell line be accessed? | |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Federal Commission Medical and Scientific Research on embryos in vitro (Belgium) |
| Approval number |
hESC Derivation
| Date of derivation | 2007-07-16 |
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
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| PGD Embryo? |
Yes |
| ZP removal technique | Enzymatic |
| Cell isolation | Mechanical |
| Cell seeding | Single cell and ICM isolated |
Culture Conditions
| Feeder cells |
Mouse Embryonic Fibroblast Cell Cellfinder Ont Id: EFO_0004040 |
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| O2 Concentration | 20 % | |||||||||
| CO2 Concentration | 10 % | |||||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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| DNMT3B |
Yes |
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| GDF3 |
Yes |
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| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| ZFP42 (REX-1) |
Yes |
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| SOX2 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XX
Passage number: 5
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Other Genotyping (Cell Line) |
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