VUB19_DM1

The cell line is not validated yet.

General

Cell Line

hPSCreg Name
VUBe019-A
Alternative name(s)
VUB19_DM1
Cell line type Human embryonic stem cell (hESC)
Last update 27th April 2022
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Provider

Generator Vrije Universiteit Brussel (VUB)
Derivation country Belgium

External Databases

Cellosaurus CVCL_9860
CLO CLO_0100253
Wikidata Q54993413

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Steinert myotonic dystrophy
The donor is affected.
Synonyms
  • Myotonic dystrophy type 1
  • Steinert disease

Ethics

Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number
How may genetic information associated with the cell line be accessed?
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Federal Commission Medical and Scientific Research on embryos in vitro (Belgium)
Approval number

hESC Derivation

Date of derivation 2007-07-16
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
Yes
ZP removal technique Enzymatic
Cell isolation Mechanical
Cell seeding Single cell and ICM isolated

Culture Conditions

Feeder cells Mouse Embryonic Fibroblast Cell
Cellfinder Ont Id: EFO_0004040
O2 Concentration 20 %
CO2 Concentration 10 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine
hrbFGF %

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
DNMT3B
Yes
GDF3
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
ZFP42 (REX-1)
Yes
SOX2
Yes
Differentiation Potency

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Passage number: 5

Other Genotyping (Cell Line)