General

iPSC Line
hPSCreg name	VUBe012-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	VUBe012-A (RRID:CVCL_9849)
Alternative name(s)	VUB12
iPSC line type	Human embryonic stem cell (hESC)
Similar iPSC lines	No similar lines found.
Last update	7th April 2022
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Provider
Generator	Vrije Universiteit Brussel (VUB) Contact: Department of Embryology and Genetics
Derivation country	Belgium
External iPSC Databases
Cellosaurus	CVCL_9849
Wikidata	Q54993398
General iPSC Information
Publications	Mateizel I et al. Derivation, culture, and characterization of VUB hESC lines. In vitro cellular & developmental biology. Animal. 2010 Apr;46(3-4):300-8. Mor-Shaked H et al. Modeling Fragile X Syndrome Using Human Pluripotent Stem Cells. Genes. 2016 Sep 28;7(10).
* Is the cell line readily obtainable for third parties?	Yes

Donor Information

General Donor Information
Sex	female
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed.

Ethics

How may genetic information associated with the cell line be accessed?
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions?	Yes
Name of accrediting authority involved?	Federal Commission Medical and Scientific Research on embryos in vitro (Belgium)
Approval number

hESC Derivation

Date of derivation	2006-09-19
Embryo stage	Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?	No Other hESC source: IVF
PGD Embryo?	Yes
Cell isolation	Mechanical
Cell seeding	Isolated ICM

iPSC Culture Conditions

Surface coating	MEF
O2 Concentration	20 %
CO2 Concentration	10 %
Medium	Other medium: Base medium: Knockout-DMEM Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements Supplement Amount Unit L-Glutamine bFGF

iPSC Characterisation

Analysis of undifferentiated iPSCs

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles

Genotyping

Karyotyping (iPSC Line)
Has the iPSC line karyotype been analysed?	Yes 46XX
Other Genotyping (iPSC Line)