VUB12
The cell line is not validated yet.
VUBe012-A
General
Cell Line |
|
| hPSCreg name | VUBe012-A |
| Cite as: | VUBe012-A (RRID:CVCL_9849) |
| Alternative name(s) |
VUB12
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 7th April 2022 |
| User feedback | |
Provider |
|
| Generator | Vrije Universiteit Brussel (VUB) |
| Derivation country | Belgium |
External Databases |
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| Cellosaurus | CVCL_9849 |
| Wikidata | Q54993398 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | A disease was diagnosed. |
Ethics
| How may genetic information associated with the cell line be accessed? | |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Federal Commission Medical and Scientific Research on embryos in vitro (Belgium) |
| Approval number |
hESC Derivation
| Date of derivation | 2006-09-19 |
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
No
Other hESC source:
IVF
|
| PGD Embryo? |
Yes |
| Cell isolation | Mechanical |
| Cell seeding | Isolated ICM |
Culture Conditions
| Surface coating | MEF | |||||||||
| O2 Concentration | 20 % | |||||||||
| CO2 Concentration | 10 % | |||||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XX
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Other Genotyping (Cell Line) |
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