VUB06
VUBe006-A
General
Cell Line |
|
| hPSCreg name | VUBe006-A |
| Cite as: | VUBe006-A (RRID:CVCL_9843) |
| Alternative name(s) |
VUB06
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 15th October 2021 |
| User feedback | |
Provider |
|
| Generator | Vrije Universiteit Brussel (VUB) |
| Derivation country | Belgium |
External Databases |
|
| Cellosaurus | CVCL_9843 |
| Wikidata | Q54993391 |
General Information |
|
| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes |
Donor Information
General Donor Information |
|
| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | |
Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Federal Commission Medical and Scientific Research on embryos in vitro (Belgium) |
hESC Derivation
| Date of derivation | 2005-05-05 |
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
No |
| Cell isolation | Immunosurgery |
| Cell seeding | Isolated ICM |
Culture Conditions
| Feeder cells |
mouse embryonic fibroblast cell Cellfinder Ont Id: EFO_0004040 |
|||||||||
| O2 Concentration | 20 % | |||||||||
| CO2 Concentration | 10 % | |||||||||
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
|
||||
| SSEA-1 |
No |
|
||||
| SSEA-3 |
Yes |
|
||||
| SSEA-4 |
Yes |
|
||||
| TRA 1-60 |
Yes |
|
||||
| TRA 1-81 |
Yes |
|
||||
| DNMT3B |
Yes |
|
||||
| GDF3 |
Yes |
|
||||
| LIN28 |
Yes |
|
||||
| NANOG |
Yes |
|
||||
| NPM1 |
Yes |
|
||||
| POU5F1 (OCT-4) |
Yes |
|
||||
| ZFP42 (REX-1) |
Yes |
|
||||
| SOX2 |
Yes |
|
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46XX
Passage number: 64
|
Other Genotyping (Cell Line) |
|
Login to share your feedback, experiences or results with the research community.