VUB10_SCA7

General#

Cell Line

hPSCreg Name VUBe010-A
Alternative name(s)
VUB10_SCA7
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
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Provider

Generator Vrije Universiteit Brussel (VUB)
Derivation country Belgium

External Databases

Cellosaurus CVCL_9847

General Information

Publications
Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
autosomal dominant cerebellar ataxia
The donor is affected.
Synonyms
  • spinocerebellar ataxia
Disease associated phenotypes
  • SCA7 (Spinocerebellar Ataxia Type 7)

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2006-06-19
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
No
Other hESC source: IVF
PGD Embryo?
Yes
Cell isolation Immunosurgery
Cell seeding Isolated ICM

Culture Conditions#

Surface coating MEF
O2 Concentration 20 %
CO2 Concentration 10 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine
bFGF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
SSEA-1
No
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
DNMT3B
Yes
GDF3
Yes
LIN28
Yes
NANOG
Yes
NPM1
Yes
POU5F1 (OCT-4)
Yes
ZFP42 (REX-1)
Yes
SOX2
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)