INSRMe010-A

STR-I-221-Sca2

General#

Cell Line

hPSCreg Name INSRMe010-A
Alternative name(s)
STR-I-221-Sca2
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
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Provider

Generator INSERM (INSRM)
Derivation country France

External Databases

Cellosaurus CVCL_C088

General Information

Publications
Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
autosomal dominant cerebellar ataxia
The donor is affected.
Synonyms
  • spinocerebellar ataxia
Disease associated phenotypes
  • Spinocerebellar ataxia type 2

Ethics#

We are currently revising this section.

hESC Derivation#

Embryo stage Blastula with ICM and Trophoblast
PGD Embryo?
Yes
ZP removal technique Enzymatic

Culture Conditions#

Surface coating Gelatin
Passage method Mechanically
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
L-Glutamine
bFGF

Characterisation#

Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)