HESC-NL4

General#

Cell Line

hPSCreg Name LUMCe004-A
Alternative name(s)
HESC-NL4
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
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Provider

Generator Leiden University Medical Center (LUMC)
Derivation country Netherlands

External Databases

Cellosaurus CVCL_C780

General Information

Publications
Is the cell line available in principle?
Yes

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases

Ethics#

We are currently revising this section.

hESC Derivation#

Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No

Culture Conditions#

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
GCTM2
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
SSEA-3
Yes
TRA 1-60
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro directed differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY

Other Genotyping (Cell Line)