Amicqui-1, Amicqui1, Amiqui1
INPERe001-A
General
Cell Line |
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| hPSCreg name | INPERe001-A |
| Cite as: | INPERe001-A (RRID:CVCL_UL74) |
| Alternative name(s) |
Amicqui-1, Amicqui1, Amiqui1
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| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 1st November 2023 |
| User feedback | |
Provider |
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| Generator | Instituto Nacional de Perinatología (INPER) |
External Databases |
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| Cellosaurus | CVCL_UL74 |
| Wikidata | Q94318017 |
| BioSamples | SAMEA114245580 |
General Information |
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| Publications |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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External Databases (Donor) |
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| BioSamples | SAMEA14409934 |
Ethics
| Was the embryo established purely for research purposes? | No |
| Have both parents consented to the use of the embryo for ESC derivation? | Yes |
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | No information |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Ethics Research Committee of the National Institute of Perinatology |
| Approval number | INPER 212250-21071 |
hESC Derivation
| Date of derivation | 2014-12-01 |
| Supernumerary embryos from IVF treatment? |
Yes
Separation of research and IVF treatment?
Yes |
| PGD Embryo? |
No |
Culture Conditions
| Feeder cells |
human amniotic epithelial cells |
| Medium |
Other medium:
Base medium: DMEM/F-12
Main protein source: Knock-out serum replacement Serum concentration: % |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| TRA 1-60 |
Yes |
Score: 0.38
| Marker | Present | Absent |
| mCpG | X | |
| OCT4 | X |
Morphology pictures
10x_Amicqui1-iMEF.tif
Representative phase-contrast micrograph of Amicqui-1 hESC on iMEF feeder layer (10X)
Amicqui1-CMhAEC.tif
Representative micrograph for Amicqui-1 on Matrigel with conditioned media of human amniotic epithelial cell (hAEC)
Amicqui1-hAEC.tif
Representative phase-contrast micrograph of Amicqui-1 hESC on human amniotic epithelial cells (hAEC) feeder layer
Transcriptome Characterisation
Differentiation Potency
In vivo teratoma
In vitro spontaneous differentiation
In vivo teratoma
In vitro spontaneous differentiation
| Marker | Expressed |
| T-box transcription factor T |
Yes |
In vivo teratoma
In vitro spontaneous differentiation
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46,XY
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Other Genotyping (Cell Line) |
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