IBMS-iPSC-014-05
IBMSi003-A
General#
Cell Line |
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hPSCreg Name | IBMSi003-A |
Alternative name(s) |
IBMS-iPSC-014-05
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Last update | 8th December 2017 |
User feedback | |
Provider |
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Generator | Institute of Biomedical Sciences, Academia Sinica (IBMS) |
External Databases |
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BioSamples | SAMEA104387752 |
Cellosaurus | CVCL_QY07 |
General Information |
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Publications | |
Is the cell line available in principle? | Available with restrictions: Available for non-commercial research |
Donor Information#
General Donor Information |
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Sex | female |
Ethnicity | East Asian (Taiwanese) |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
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External Databases (Donor) |
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BioSamples | SAMEA104387753 |
Ethics#
We are currently revising this section.hIPSC Derivation#
General |
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Source cell type |
peripheral blood mononuclear cellA leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.
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Age of donor (at collection) | 60-64 |
Reprogramming method |
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Vector type | Non-integrating |
Vector | Sendai virus |
Is reprogramming vector detectable? |
No |
Methods used |
PCR
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Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
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Other |
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Selection criteria for clones | Tra-1-60 positive, ES-like morphology |
Derived under xeno-free conditions |
Unkown |
Derived under GMP? |
Unkown |
Available as clinical grade? |
Unkown |
Culture Conditions#
Medium |
Other medium:
Base medium: DMEM/F12
Main protein source: Knock-out serum replacement Serum concentration: 20 % Supplements
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
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Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
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Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
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SOX2 |
Unkown |
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NANOG |
Unkown |
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SSEA-4 |
Unkown |
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TRA 1-60 |
Unkown |
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SSEA-3 |
Unkown |
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TRA 1-81 |
Unkown |
Score:
Marker | Present | Absent |
mCpG | ||
OCT4 |
Differentiation Potency
Glandular structure
In vivo teratoma
Neural tissue
In vivo teratoma
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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