IBMS-iPSC-014-05

General

Cell Line

hPSCreg name IBMSi003-A
Cite as:
IBMSi003-A (RRID:CVCL_QY07)
Alternative name(s)
IBMS-iPSC-014-05
Cell line type Human induced pluripotent stem cell (hiPSC)
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Last update 22nd May 2019
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Provider

Generator Institute of Biomedical Sciences, Academia Sinica (IBMS)

External Databases

BioSamples SAMEA104387752
Cellosaurus CVCL_QY07
Wikidata Q54897192

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclones

Donor Information

General Donor Information

Sex female
Age of donor (at collection) 60-64
Ethnicity East Asian (Taiwanese)

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Polycystic kidneys - adult type
  • Autosomal dominant adult polycystic kidney disease
  • ADPKD
  • adult type autosomal dominant polycystic kidney disease
  • Polycystic kidney disease, adult type (disorder)
  • Autosomal dominant polycystic kidney disease
  • polycystic kidney disease, adult type
  • ADPKD - Autosomal dominant polycystic kidney disease
  • polycystic kidney, autosomal dominant
  • polycystic kidney disease, autosomal dominant
  • autosomal dominant polycystic kidney disease
  • congenital biliary ectasias
show more synonyms
Genetic variants
chr4: 88989098C>T, p.Arg803*

External Databases (Donor)

BioSamples SAMEA104387753

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. michellelee@ibms.sinica.edu.tw
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information michellelee@ibms.sinica.edu.tw
Alternatives to consent
Alternative consent approval number
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Details on restriction to research project
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Please describe how access is provided:
Contact data, institution, or website:
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Institution Review Board on biomedical science research at Academia Sinica Taiwan and Kaohsiung Medical University Hospital
Approval number (AS-IRB-BM-15019) (KMUHIRB-20140116)
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? No
Name of accrediting authority involved?
Approval number
Please describe:
Further constraints on use
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Constraints for use or distribution

hIPSC Derivation

General

Source cell type
A leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.
Age of donor (at collection) 60-64

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Selection criteria for clones Tra-1-60 positive, ES-like morphology
Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium Other medium:
Base medium: DMEM/F12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
NEAA 0.1 mM
Glutamine 1 mM
2-mercaptoethanol 0.1 mM
bFGF 10 ng/ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
Score:
Marker Present Absent
mCpG
OCT4
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Glandular structure
In vivo teratoma
Cartilage Element
Ont Id: UBERON_0007844
In vivo teratoma
Neural tissue
In vivo teratoma

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)