IBMS-iPSC-014-05

General#

Cell Line

hPSCreg Name IBMSi003-A
Alternative name(s)
IBMS-iPSC-014-05
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th December 2017
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Provider

Generator Institute of Biomedical Sciences, Academia Sinica (IBMS)

External Databases

BioSamples SAMEA104387752
Cellosaurus CVCL_QY07

General Information

Publications
Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex female
Ethnicity East Asian (Taiwanese)

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Autosomal dominant polycystic kidney disease
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Polycystic kidneys - adult type
  • Autosomal dominant adult polycystic kidney disease
  • ADPKD
  • adult type autosomal dominant polycystic kidney disease
  • Polycystic kidney disease, adult type (disorder)
  • Autosomal dominant polycystic kidney disease
  • polycystic kidney disease, adult type
  • ADPKD - Autosomal dominant polycystic kidney disease
  • polycystic kidney, autosomal dominant
show more synonyms
Genetic variants
chr4: 88989098C>T, p.Arg803*

External Databases (Donor)

BioSamples SAMEA104387753

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
peripheral blood mononuclear cell
A leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.
Age of donor (at collection) 60-64

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Selection criteria for clones Tra-1-60 positive, ES-like morphology
Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Medium Other medium:
Base medium: DMEM/F12
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
NEAA 0.1 mM
Glutamine 1 mM
2-mercaptoethanol 0.1 mM
bFGF 10 ng/ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Unkown
NANOG
Unkown
SSEA-4
Unkown
TRA 1-60
Unkown
SSEA-3
Unkown
TRA 1-81
Unkown
Score:
Marker Present Absent
mCpG
OCT4
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Glandular structure
In vivo teratoma
Cartilage Element
Ont Id: UBERON_0007844
In vivo teratoma
Neural tissue
In vivo teratoma

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)