SCVI2617
SCVIi091-A
General
Cell Line |
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| hPSCreg name | SCVIi091-A |
| Cite as: | SCVIi091-A (RRID:CVCL_D0LB) |
| Alternative name(s) |
SCVI2617
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
FMMUNIi003-A (I-CPVT) Donor's gene variants: RYR2 Donor diseases: catecholaminergic polymorphic ventricular tachycardia ZJSHi001-A (ZJSHi-KCNB1) Donor's gene variants: KCNB1 Donor diseases: developmental and epileptic encephalopathy, 26 IMBAi021-A (TSC107J #5, TSC#14 clone 107J#5) Donor's gene variants: TSC2, TSC2 Donor diseases: Tuberous Sclerosis IMBAi022-A (TSC112#4, TSC#24 clone 112#4) Donor's gene variants: TSC2, TSC2 Donor diseases: Tuberous Sclerosis IMBAi023-A (TSC#5 clone 32S, TSCp5#32S) Donor's gene variants: TSC2, TSC2 Donor diseases: Tuberous Sclerosis IMBAi023-B (TSC#5 clone 30S, TSCp5#30S, TSC98#30S) Donor's gene variants: TSC2, TSC2, TSC2 Donor diseases: Tuberous Sclerosis |
| Last update | 4th October 2023 |
| User feedback | |
Provider |
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| Generator | Stanford Cardiovascular Institute (SCVI) |
External Databases |
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| BioSamples | SAMEA113567256 |
| Cellosaurus | CVCL_D0LB |
| Wikidata | Q123033434 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
|
| Diseases | A disease was diagnosed.
|
External Databases (Donor) |
|
| BioSamples | SAMEA113567257 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | Yes |
| How may genetic information associated with the cell line be accessed? | Controlled Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | |
| Approval number | |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
|
| Source cell type |
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
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Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Medium |
StemPro® hESC SFM
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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Differentiation Potency
In vitro directed differentiation
| Marker | Expressed |
| SOX17 |
Yes |
| FOXA2 |
Yes |
In vitro directed differentiation
| Marker | Expressed |
| Brachyury |
Yes |
| TBX6 |
Yes |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
Normal
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Other Genotyping (Cell Line) |
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