General

Cell Line

hPSCreg Name
SCVIi077-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 12th August 2023
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Provider

Generator Stanford Cardiovascular Institute (SCVI)
Owner Stanford Cardiovascular Institute (SCVI)
Distributors
Derivation country United States

External Databases

Cellosaurus CVCL_C7YF
BioSamples SAMEA114260323

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex female
Ethnicity Hispanic or Latino

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
catecholaminergic polymorphic ventricular tachycardia
Family history No family history of cardiac arrhythmias

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes

External Databases (Donor)

BioSamples SAMEA114260325

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: yanzhuge@stanford.edu
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Peripheral Blood Mononuclear Cell
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • Peripheral Blood Mononuclear cells
  • PBMC
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
CO2 Concentration 5 %
Medium StemPro® hESC SFM

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SOX2
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
Morphology pictures
Immunostaining of three pluripotency markers: SOX2, NANOG, OCT3/4
Method documentation
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Morphology

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
CN=2, Female normal karyotype
Passage number: 12

Other Genotyping (Cell Line)