NP0014-6, UKK007Ai

General#

Cell Line

hPSCreg Name UKKi007-A
Alternative name(s)
NP0014-6, UKK007Ai
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 13th March 2019
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Provider

Generator Klinikum der Universität zu Köln (UKK)
Owner Institute for Neurophysiology, Medical Faculty
Distributors

External Databases

BioSamples SAMEA2629464
Cellosaurus CVCL_2Z94
ECACC 66540001
EBiSC UKKi007-A

General Information

Publications
Projects
Is the cell line available in principle? Available, without restrictions

Donor Information#

General Donor Information

Sex female
Ethnicity Caucasian, German

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Catecholaminergic polymorphic ventricular tachycardia
CPVT1, Mutation in RYR2 gene c.7447T>A, p.F2483I
The donor is a carrier of a disease-associated mutation and affected.
Stage
Symptomatic
Synonyms
  • Double tachycardia induced by catecholamines
  • CPVT
  • Bidirectional tachycardia induced by catecholamine
  • Malignant paroxysmal ventricular tachycardia
  • Multifocal ventricular premature beats
show more synonyms
Disease associated phenotypes
  • Catecholaminergic polymorphic ventricular tachycardia (CPVT) type 1 [CPVT1, point mutation p.F2483I, c7447T>A (Phe > Ile) in RYR2 gene]
  • Cardiac arrhythmia at exercise; normal ECG at rest
Family history de novo mutation
Is the medical history available upon request? No (can be retreived upon request)
Is clinical information available? Not available

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unkown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA2629512

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Provide contact information of the holder of the original Donor Information Sheet:
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? Yes
Contact information / weblink
Alternatives to consent
Has the donor been informed about how her/his data will be protected? Yes
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent pertain to a specific research project? No
Details on restriction to research project
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
an academic institution? Yes
Does consent expressly permit collection of genetic information? Yes
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? Yes
Policy for access to genetic information
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? No
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? Yes
Does consent permit access to medical records of the donor? Yes
Please describe how access is provided:
Contact data, institution, or website:
Name of accrediting authority involved? Ethics Committee of the Ruhr-University Bochum in Bad Oeynhausen
Approval number 41/2008
Name of accrediting authority involved? Ethics Committee of the Ruhr-University Bochum in Bad Oeynhausen
Approval number 41/2008
Do you have obligations to third parties in regard to the use of the cell line? No
Please describe:
Further constraints on use

hIPSC Derivation#

General

Source cell type
fibroblast of dermis
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Age of donor (at collection) 45-49
Collected in 2009
Passage number reprogrammed P3

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
No
Reprogramming vectors silenced?
Yes
Methods used
RT-PCR
Notes on reprogramming vector silencing Retrovirally encoded reprogramming factors OCT4, KLF4 and cMYC are not expressed as determined by RT-PCR. Only Sox2 seems to be expressed. at very low levels.
Files and images showing reprogramming vector expressed or silenced
Vector map

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones human ESC morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Unkown
SSEA-4
Yes
TRA 1-60
Unkown
ZFP42 (REX-1)
Unkown
TRA 1-80
Yes
Morphology pictures
Brightfield image of NP0014-6 iPSC colony on day 4 post-thaw at higher magnification; 32x objective
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Not done
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Karyotyping method: Molecular karyotyping by SNP array

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
SNP genotyping using OmniExpress Exome Chip