TRDS-Cardio-021
TRDSi001-A
General#
Cell Line |
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hPSCreg Name | TRDSi001-A |
Alternative name(s) |
TRDS-Cardio-021
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Last update | 22nd May 2019 |
User feedback | |
Provider |
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Generator | Technion Research and Development Foundation (TRDS) |
External Databases |
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BioSamples | SAMEA104620783 |
Cellosaurus | CVCL_VF05 |
CLO | CLO_0101715 |
General Information |
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* Is the cell line readily obtainable for third parties? |
No |
Donor Information#
General Donor Information |
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Sex | male |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
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External Databases (Donor) |
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BioSamples | SAMEA104620784 |
Ethics#
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
Was the consent voluntarily given? | Yes |
Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
Do you (Depositor/Provider) hold a copy of the Donor Consent Form? | No |
If you do not hold the Donor Consent Form, do you know who does? | No |
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? | Yes |
Alternatives to consent | |
Alternative consent approval number | |
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
Does consent expressly prevent the derivation of pluripotent stem cells? | No |
Details on restriction to research project | |
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
How may genetic information associated with the cell line be accessed? | No information |
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
Please describe how access is provided: | |
Contact data, institution, or website: | |
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
Name of accrediting authority involved? | Helsinki Committee of the Rambam Health Care Campus, Haifa, Israel |
Approval number | 0170-09-RMB |
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | Yes |
Name of accrediting authority involved? | Helsinki Committee of the Rambam Health Care Campus, Haifa, Israel |
Approval number | 0170-09-RMB |
Please describe: | |
Further constraints on use | |
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
Constraints for use or distribution |
hIPSC Derivation#
General |
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Source cell type |
fibroblastA connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
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Source cell type (free text) | Dermal fibroblasts from skin biopsy |
Reprogramming method |
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Vector type | Integrating |
Vector | Virus (Retrovirus) |
Genes | |
Is the used vector excisable? |
No |
Absence of reprogramming vector(s)? |
Unknown |
Reprogramming vectors silenced? | |
Vector free reprogramming |
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Other |
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Derived under xeno-free conditions |
Unknown |
Derived under GMP? |
Unknown |
Available as clinical grade? |
Unknown |
Culture Conditions#
Medium |
mTeSR™ 1
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Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
TRA 1-60 |
Yes |
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POU5F1 (OCT-4) |
Yes |
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Differentiation Potency
In vitro spontaneous differentiation
In vitro directed differentiation
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
Normal
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Other Genotyping (Cell Line) |
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