TRDS-Cardio-021
TRDSi001-A
General#
Cell Line |
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hPSCreg Name | TRDSi001-A |
Alternative name(s) |
TRDS-Cardio-021
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Last update | 8th December 2017 |
User feedback | |
Provider |
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Generator | Technion Research and Development Foundation (TRDS) |
External Databases |
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BioSamples | SAMEA104620783 |
Cellosaurus | CVCL_VF05 |
General Information |
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Is the cell line available in principle? |
No |
Donor Information#
General Donor Information |
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Sex | male |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
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External Databases (Donor) |
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BioSamples | SAMEA104620784 |
Ethics#
We are currently revising this section.hIPSC Derivation#
General |
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Source cell type |
fibroblastA connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
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Source cell type (free text) | Dermal fibroblasts from skin biopsy |
Reprogramming method |
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Vector type | Integrating |
Vector | Virus (Retrovirus) |
Genes | |
Is the used vector excisable? |
No |
Absence of reprogramming vector(s)? |
Unkown |
Reprogramming vectors silenced? | |
Vector free reprogramming |
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Other |
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Derived under xeno-free conditions |
Unkown |
Derived under GMP? |
Unkown |
Available as clinical grade? |
Unkown |
Culture Conditions#
Medium |
mTeSR™ 1
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Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
TRA 1-60 |
Yes |
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POU5F1 (OCT-4) |
Yes |
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SOX2 |
Unkown |
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SSEA-4 |
Unkown |
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NANOG |
Unkown |
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Differentiation Potency
In vitro spontaneous differentiation
In vitro directed differentiation
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
Normal
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Other Genotyping (Cell Line) |
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