NP0014-5
UKKi007-B
General#
Donor Information#
General Donor Information |
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Sex | female |
Age of donor (at collection) | 45-49 |
Ethnicity | Caucasian, German |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
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Disease associated phenotypes |
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Non-disease associated phenotypes |
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Family history | de novo mutation |
Is the medical history available upon request? | No (can be retreived upon request) |
Is clinical information available? | Not available |
Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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Other cell lines of this donor | |
External Databases (Donor) |
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BioSamples | SAMEA2629512 |
Ethics#
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? | No |
If you do not hold the SIGNED Donor Consent Form, do you know who does? | No |
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? | Yes |
Has the donor been informed about how her/his data will be protected? | Yes |
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
Does consent pertain to a specific research project? | No |
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? | No |
Does consent permit research by | |
an academic institution? | Yes |
Does consent expressly permit collection of genetic information? | Yes |
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? | Yes |
How may genetic information associated with the cell line be accessed? | |
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? | No |
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? | Yes |
Does consent permit access to medical records of the donor? | Yes |
Please describe how access is provided: | |
Do you have obligations to third parties in regard to the use of the cell line? | No |
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation#
General |
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Source cell type |
fibroblast of dermis |
Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
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Age of donor (at collection) | 45-49 |
Collected in | 2009 |
Passage number reprogrammed | P3 |
Reprogramming method |
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Vector type | Integrating |
Vector | Virus (Retrovirus) |
Genes | |
Is the used vector excisable? |
No |
Absence of reprogramming vector(s)? |
Unknown |
Reprogramming vectors silenced? | |
Vector map | |
Vector free reprogramming |
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Type of used vector free reprogramming factor(s) |
None
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Other |
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Selection criteria for clones | human ESC morphology |
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
Surface coating | Vitronectin |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
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O2 Concentration | 20 % |
CO2 Concentration | 5 % |
Medium |
Essential 8™
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Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
NANOG |
Yes |
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POU5F1 (OCT-4) |
Yes |
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SSEA-4 |
Yes |
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TRA1-80 |
Yes |
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Differentiation Potency
In vitro spontaneous differentiation
Marker | Expressed |
HAND1 |
Yes |
VIMENTIN |
Yes |
BMP4 |
Yes |
GATA4 |
No |
DCN |
Yes |
PECAM1 (CD31) |
Yes |
PDGF |
Yes |
In vitro spontaneous differentiation
Marker | Expressed |
SOX17 |
Yes |
FOXA2 |
No |
GSC |
Yes |
GATA6 |
Yes |
CXCR4 |
Yes |
PITX1 |
Yes |
MYH6, alpha myosin heavy chain |
Yes |
MLC2v, myosin light chain 2v |
Yes |
ACTN2, cardiac alpha actinin |
Yes |
RYR2 |
Yes |
In vitro spontaneous differentiation
Marker | Expressed |
PAX6 |
Yes |
Sox1 |
Yes |
B-TUBULIN |
Yes |
NEUROD1 |
Yes |
HES5 |
Yes |
FOXG1 |
No |
Microbiology / Virus Screening |
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HIV 1 | Negative |
HIV 2 | Not done |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Certificate of Analysis |
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Is there a certificate of analysis available? |
Yes
Passage:
33
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Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
XY
Karyotyping method:
Molecular karyotyping using using OmniExpress Exome Chip
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Other Genotyping (Cell Line) |
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Is there genome-wide genotyping or functional data available? |
Yes
SNP-genotyping using OmniExpress Exome Chip
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