LB-33-5, iLB-MJD3-33f-r5

General

Cell Line

hPSCreg name UKBi007-A
Cite as:
UKBi007-A (RRID:CVCL_W574)
Alternative name(s)
LB-33-5, iLB-MJD3-33f-r5
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
UKBi003-A
(iLB-MJD1-32m-r9, LB-32-r9)
Donor's gene variants:
ATXN3, ATXN3
Donor diseases:
Machado-Joseph disease
UKBi008-A
(iLB-MJD4-34m-r1, LB-34-1)
Donor's gene variants:
ATXN3, ATXN3
Donor diseases:
Machado-Joseph disease
UKBi001-B
Donor's gene variants:
ATXN3
Donor diseases:
Machado-Joseph disease
ZZUi014-A
(ZZU-iPS-SCA3-002)
Donor diseases:
Spinocerebellar ataxia type 3
ZZUi004-A
(ZZU-iPS-SCA3-001)
Donor diseases:
Spinocerebellar ataxia type 3
ZZUi026-A
(ZZU-iPS-SCA3-003)
Donor diseases:
Spinocerebellar Ataxia Type 3
RCi004-A
(PDSC-10, RCi150)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
RCi004-B
(RCi68, PDSC-3)
Donor's gene variants:
HTT, HTT
Donor diseases:
Huntington disease
CSSi002-A
(HD8yrs)
Donor diseases:
Juvenile Huntington disease
NMIi004-A
Donor diseases:
Schizophrenia
SHEHDNi002-A
(KY03AP)
Donor's gene variants:
LRRK2, DNAJC6
Donor diseases:
Parkinson Disease
NMIi006-A
Donor diseases:
Schizophrenia
NMIi006-B
Donor diseases:
Schizophrenia
ESi060-A
([SWB] FiPS-4F-5-6)
Donor diseases:
Williams syndrome
EDi001-A
(AST22, AST23, SAMEA3319992)
Donor's gene variants:
SNCA, SNCA, SNCA
Donor diseases:
Parkinson disease
UOXFi001-A
(MK071-1)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
UOXFi001-B
(MK071-3)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
UOXFi001-C
(MK071-5)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
UOXFi001-D
(MK071-6)
Donor's gene variants:
GBA1
Donor diseases:
Parkinson disease
STBCi006-A
(SFC140-04-01)
Donor's gene variants:
APOE, APOE
Donor diseases:
Alzheimer disease
UCLi003-A
(TSM(exon10+16)V97)
Donor's gene variants:
MAPT
Donor diseases:
Frontotemporal dementia
UGOTSAi004-A
(N1-003iC3)
Donor diseases:
Alzheimer's Disease
UGOTSAi004-B
(N1-003iC4)
Donor diseases:
Alzheimer's Disease
UCLi009-B
(CB1E)
Donor's gene variants:
VCP
Donor diseases:
Frontotemporal dementia
UOXFi007-A
(MK002-4)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
ESi100-A
(NW FiPS 10II.3-R4F-1)
Donor diseases:
Williams Syndrome
UOXFi008-A
(MK144-1)
Donor's gene variants:
LRRK2
Donor diseases:
Parkinson disease
EDi001-B
(AST18)
Donor's gene variants:
SNCA
Donor diseases:
Parkinson disease
CSSi001-A
(Joub03cl2, COR419)
Donor diseases:
Joubert syndrome
UNEWi022-A
(F181 5.8)
Donor's gene variants:
CFH
Donor diseases:
age-related macular degeneration
UNEWi024-A
(F180-1)
Donor's gene variants:
CFH
Donor diseases:
age-related macular degeneration
UNEWi022-B
(F181 18.2)
Donor's gene variants:
CFH
Donor diseases:
age-related macular degeneration
UNEWi022-C
(F181 25.7)
Donor's gene variants:
CFH
Donor diseases:
age-related macular degeneration
UNEWi026-A
(SF116 clone 1)
Donor's gene variants:
CFH
Donor diseases:
type 2 diabetes mellitus
age-related macular degeneration
UNEWi024-B
(F180-2)
Donor's gene variants:
CFH
Donor diseases:
age-related macular degeneration
UNEWi024-C
(F180-3)
Donor's gene variants:
CFH
Donor diseases:
age-related macular degeneration
UNEWi026-B
(SF116 clone 2)
Donor's gene variants:
CFH
Donor diseases:
type 2 diabetes mellitus
age-related macular degeneration
UNEWi026-C
(SF116 clone K)
Donor's gene variants:
CFH
Donor diseases:
type 2 diabetes mellitus
age-related macular degeneration
UCLi006-A
(LCMD-L302P-UCL01C2)
Donor's gene variants:
LMNA
Donor diseases:
Congenital muscular dystrophy due to LMNA mutation
UKKi007-A
(NP0014-6, UKK007Ai)
Donor's gene variants:
RYR2, RYR2
Donor diseases:
Catecholaminergic polymorphic ventricular tachycardia
UKKi007-B
(NP0014-5)
Donor's gene variants:
RYR2
Donor diseases:
Catecholaminergic polymorphic ventricular tachycardia
UCLi002-A
(HHItC9D-V34, DN19)
Donor's gene variants:
C9orf72, C9orf72
Donor diseases:
Frontotemporal dementia
CSSi004-A
(HD256.05 cl1)
Donor diseases:
Huntington disease
Last update 4th July 2024
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Provider

Generator Universitätsklinikum Bonn (UKB)
Owner Universitätsklinikum Bonn
Distributors

External Databases

BioSamples SAMEA2825788
Cellosaurus CVCL_W574
Wikidata Q54905793

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: allowed

Donor Information

General Donor Information

Sex female
Age of donor (at collection) 40-44
Ethnicity Caucasian, German

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Stage
Score on the Scale for the Assessment and Rating of Ataxia (SARA, range: 0 – 40) was 12
Synonyms
  • Azorean disease
  • MJD
  • SCA3
  • spinocerebellar ataxia 3
  • spinocerebellar ataxia type 3
show more synonyms
Genetic variants
ATXN3 (target)
14q32.12
Heterozygous
PMID: 22113611
ATXN3: CAG repeat length of 73/27
Family history de novo mutation

External Databases (Donor)

BioSamples SAMEA2825825

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly prevent development of commercial products? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? No
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? Yes
Does consent expressly permit collection of genetic information? Yes
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? Yes
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? Yes
Does consent permit access to medical records of the donor? No
Does consent permit access to any other source of information about the clinical treatment or health of the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethik-Komission Rheinische Friederich-Wilhelms-Universität - Medizinische Fakultät
Approval number 275/08
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Ethik-Komission Rheinische Friederich-Wilhelms-Universität - Medizinische Fakultät
Approval number 275/08
Do you have obligations to third parties in regard to the use of the cell line? Yes
Please describe: n/a
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? Yes
Further constraints on use must be tracked; proband person may wish to destroy all the matericel and data in case of withdrawal.
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Any skin fibroblast that is part of some dermis.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 40-44
Collected in 2008
Passage number reprogrammed P4

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Genes
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
Yes
Reprogramming vectors silenced?
Yes
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Morphology
Neuroepithelial Stem Cell
Ont Id: CL_0002259
In vivo teratoma
In vitro directed differentiation
Morphology

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage: 13

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
XX
Passage number: 11
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes