ZZU-iPS-SCA3-001

General#

Cell Line

hPSCreg Name ZZUi004-A
Alternative name(s)
ZZU-iPS-SCA3-001
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th December 2017
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Provider

Generator Zheng Zhou University - The first affiliated hospital (ZZU)
Owner Zheng Zhou University - The first affiliated hospital (ZZU)
Derivation country China

External Databases

BioSamples SAMEA104388090
Cellosaurus CVCL_LE97

General Information

Publications
Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex female
Ethnicity Han

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Spinocerebellar ataxia type 3
The donor is a carrier of a disease-associated mutation and affected.
Stage
The patient suffered from SCA3 for 3 years.
Synonyms
  • Nigro-spino-dentatal degeneration with nuclear ophthalmoplegia
  • Machado-Joseph disease
  • SCA3
  • MJD
  • Machado disease
  • Azorean disease of the nervous system
show more synonyms

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46XX
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA104388091

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
Kidney Tubules
Source cell origin
urine
Excretion that is the output of a kidney
Source cell type (free text) Kidney tubules epithelial cells
Age of donor (at collection) 30-34
Collected in 2016
Passage number reprogrammed P3

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR
Notes on reprogramming vector detection Reprogramming vector can't be detectable on passage 15.
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones These colonies will consist of iPSCs, which exhibit a hESC-like morphology characterized by a flatter cobblestone-like appearance with individual cells clearly demarcated from each other in the colonies.
Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
O2 Concentration 95 %
CO2 Concentration 5 %
Medium TeSR™ E8™
Supplements
20x supplement 25 ml
500x supplement 1 ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
AP staining of the cell line is positive.
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
GATA4
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
Brachyury
Yes
Protocol or reference
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
TUBB3
Yes

Microbiology / Virus Screening

HIV 1 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)