ZZU-iPS-SCA3-001

General

Cell Line

hPSCreg name ZZUi004-A
Cite as:
ZZUi004-A (RRID:CVCL_LE97)
Alternative name(s)
ZZU-iPS-SCA3-001
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
UKBi007-A
(LB-33-5, iLB-MJD3-33f-r5)
Donor's gene variants:
ATXN3, ATXN3
Donor diseases:
Machado-Joseph disease
ZZUi014-A
(ZZU-iPS-SCA3-002)
Donor diseases:
Spinocerebellar ataxia type 3
UKBi008-A
(iLB-MJD4-34m-r1, LB-34-1)
Donor's gene variants:
ATXN3, ATXN3
Donor diseases:
Machado-Joseph disease
UKBi003-A
(iLB-MJD1-32m-r9, LB-32-r9)
Donor's gene variants:
ATXN3, ATXN3
Donor diseases:
Machado-Joseph disease
ZZUi026-A
(ZZU-iPS-SCA3-003)
Donor diseases:
Spinocerebellar Ataxia Type 3
UKBi001-B
Donor's gene variants:
ATXN3
Donor diseases:
Machado-Joseph disease
Last update 18th October 2019
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Provider

Generator The first affiliated hospital (ZZU)
Owner The first affiliated hospital (ZZU)
Derivation country China

External Databases

BioSamples SAMEA104388090
Cellosaurus CVCL_LE97
Wikidata Q54996166

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex female
Age of donor (at collection) 30-34
Ethnicity Han

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Stage
The patient suffered from SCA3 for 3 years.
Synonyms
  • Nigro-spino-dentatal degeneration with nuclear ophthalmoplegia
  • Machado-Joseph disease
  • SCA3
  • MJD
  • Machado disease
  • Azorean disease of the nervous system
show more synonyms

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46XX
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA104388091

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Yes
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly prevent development of commercial products? Yes
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? Yes
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? Yes
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? No
a for-profit corporation? No
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? Yes
Does consent prevent dissemination of genetic information? Yes
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? Yes
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? Yes
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? Yes
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? Yes
Does consent permit access to medical records of the donor? No
Does consent permit access to any other source of information about the clinical treatment or health of the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Medical Research Ethics Committee of The First Affiliated Hospital of Zhengzhou University
Approval number 2016-007
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Medical Research Ethics Committee of The First Affiliated Hospital of Zhengzhou University
Approval number 2016-007
Do you have obligations to third parties in regard to the use of the cell line? Yes
Please describe: School of Life Sciences of Zhengzhou University have obligations in regard to the use of the cell line.
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? Yes
Further constraints on use This material and derived cells can't be used for commercial purpose or clinical transplant..
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? STEMCELL Technologies Inc
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? Yes
Constraints for use or distribution All the resources will be only used for non-commercial scientific research, instead of any other purposes.

hIPSC Derivation

General

Source cell type
Source cell origin
Excretion that is the output of a kidney.
Source cell type (free text) Kidney tubules epithelial cells
Age of donor (at collection) 30-34
Collected in 2016
Passage number reprogrammed P3

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR
Notes on reprogramming vector detection Reprogramming vector can't be detectable on passage 15.
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones These colonies will consist of iPSCs, which exhibit a hESC-like morphology characterized by a flatter cobblestone-like appearance with individual cells clearly demarcated from each other in the colonies.
Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
O2 Concentration 95 %
CO2 Concentration 5 %
Medium TeSR™ E8™
Supplements
20x supplement 25 ml
500x supplement 1 ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
AP staining of the cell line is positive.
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
GATA4
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
Brachyury
Yes
Protocol or reference
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
TUBB3
Yes

Microbiology / Virus Screening

HIV 1 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Passage number: P12
Karyotyping method: G-Banding

Other Genotyping (Cell Line)