FMMUNIi003-A

I-CPVT

General

Cell Line

hPSCreg name FMMUNIi003-A
Cite as:
FMMUNIi003-A
Alternative name(s)
I-CPVT
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 21st July 2024
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Provider

Generator Faculty of Medicine, Masaryk University (FMMUNI)
Owner Masaryk University (MUNI)
Distributors
Derivation country Czech Republic

External Databases

BioSamples SAMEA115853744

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex female
Age of donor (at collection) 50-54
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • CPVT
  • bidirectional tachycardia induced by catecholamine
  • catecholaminergic polymorphic ventricular tachycardia
  • double tachycardia induced by catecholamines
  • malignant paroxysmal ventricular tachycardia
  • multifocal ventricular premature beats
  • ventricular tachycardia, catecholaminergic polymorphic
  • catecholamine-induced polymorphic ventricular tachycardia
  • familial polymorphic ventricular tachycardia
  • polymorphic catecholergic ventricular tachycardia
  • stress-induced polymorphic ventricular tachycardia
  • syncopal paroxysmal tachycardia
show more synonyms
Genetic variants
RYR2 (target)
RCV001233711

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA115853745

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Unknown
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? Yes
Details on restriction to research project Clinically relevant biochemical, immunological, cellular biomarkers
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly prevent development of commercial products? Yes
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? Yes
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? No
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
a public organisation? Yes
a non-profit company? Yes
a for-profit corporation? No
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? Yes
Does consent prevent dissemination of genetic information? No
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? Yes
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? No
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? No
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? No
Does consent permit access to medical records of the donor? No
Does consent permit access to any other source of information about the clinical treatment or health of the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? The University Hospital Brno Ethics Committee
Approval number 30-170621/EK
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? The University Hospital Brno Ethics Committee
Approval number 30-170621/EK
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? Epi5™ Episomal iPSC Reprogramming Kit (cat.:A15960; ThermoFisher)
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General

Source cell type
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • PBMC
Source cell origin
Blood that has not been separated into its various components; blood that has not been modified except for the addition of an anticoagulant.
Synonyms
  • WHOLE BLOOD
  • Whole Blood
  • Peripheral Whole Blood
  • Whole blood
show more synonyms
Age of donor (at collection) 50-54
Collected in 2023
Passage number reprogrammed 0

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
No
Methods used
PCR
Notes on reprogramming vector detection PCR detection of Epstein-Barr virus nuclear antigen 1 (EBNA1) gene and oriP element of Epi5™ Episomal iPSC Reprogramming Kit
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Selection criteria for clones The selection criteria were morphology of colonies and cells. All tested clones have high expression of pluripotent markers
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
SOX2
Yes
TRA 1-60
Yes
Morphology pictures
Differentiation Potency
Embryoid Body
Ont Id: BTO_0001718
In vitro spontaneous differentiation
Marker Expressed
GATA6
Yes
SOX17
Yes
Morphology
Embryoid Body
Ont Id: BTO_0001718
In vitro spontaneous differentiation
Marker Expressed
MSX1
Yes
RUNX2
Yes
Morphology
Embryoid Body
Ont Id: BTO_0001718
In vitro spontaneous differentiation
Marker Expressed
SOX1
Yes
PAX6
Yes
Morphology

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Passage number: 16
Karyotyping method: G-Banding

Other Genotyping (Cell Line)