PUMCHi005-A
General
Cell Line |
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| hPSCreg name | PUMCHi005-A |
| Cite as: | PUMCHi005-A (RRID:CVCL_A9T3) |
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
ZJSHi001-A (ZJSHi-KCNB1) Donor's gene variants: KCNB1 Donor diseases: developmental and epileptic encephalopathy, 26 |
| Last update | 10th August 2020 |
| User feedback | |
Provider |
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| Generator | Peking Union Medical College Hospital (PUMCH) |
| Owner | Peking Union Medical College Hospital (PUMCH) |
| Derivation country | China |
External Databases |
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| BioSamples | SAMEA7211859 |
| Cellosaurus | CVCL_A9T3 |
| Wikidata | Q102114790 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
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| Sex | male |
| Age of donor (at collection) | 25-29 |
| Ethnicity | Han |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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| Disease associated phenotypes |
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| Family history | The patient had a clinical history of peripheral neuropathy and heart failure, familial history of paralysis and sudden cardiac death. |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
Yes
Exome sequencing
TTR: NM_000371.3: exon3: c.157T>G: p.Phe53Val: chr18:29172946 |
External Databases (Donor) |
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| BioSamples | SAMEA7211860 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | Yes |
| Confirm that consent was obtained by a qualified professional | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | Yes |
| How may genetic information associated with the cell line be accessed? | Open Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Ethics Committee of Peking Union Medical College Hospital |
| Approval number | JS-1233 |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | Yes |
| Name of accrediting authority involved? | Ethics Committee of Peking Union Medical College Hospital |
| Approval number | JS-1233 |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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| Source cell type |
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
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| Source cell origin |
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
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| Age of donor (at collection) | 25-29 |
| Collected in | 2019 |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Episomal |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
RT-PCR
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Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
None
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Other |
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| Derived under xeno-free conditions |
Yes |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex | ||||||
| Feeder cells |
No |
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| Passage method |
Enzyme-free cell dissociation
EDTA
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| O2 Concentration | 21 % | ||||||
| CO2 Concentration | 5 % | ||||||
| Medium |
Other medium:
Base medium: ncEpic basal medium
Main protein source: yeast Serum concentration: 0 % Supplements
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
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| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
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| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| OCT4 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-81 |
Yes |
Score:
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
Differentiation Potency
Microbiology / Virus Screening |
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| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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