FJMUUHi001-A
General
Cell Line |
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| hPSCreg name | FJMUUHi001-A |
| Cite as: | FJMUUHi001-A (RRID:CVCL_A1WA) |
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
CBIGi037-A (IPSC0048, LRRK2 M1646T(CBIGi037-A)) Donor's gene variants: LRRK2, LRRK2 Donor diseases: Parkinson Disease CBIGi039-A (IPSC0050, LRRK2 R1441H(CBIGi039-A)) Donor's gene variants: LRRK2, LRRK2 Donor diseases: Parkinson Disease CBIGi040-A (IPSC0052, LRRK2 N551K-R1398H-K1423K (protective haplotype) (CBIGi040-A)) Donor's gene variants: LRRK2, LRRK2 Donor diseases: Parkinson Disease CBIGi041-A (IPSC0053, GBA L444P (CBIGi041-A)) Donor's gene variants: GBA, GBA Donor diseases: Parkinson Disease CBIGi042-A (IPSC0055, GBA E326K (CBIGi042-A)) Donor's gene variants: GBA, GBA Donor diseases: Parkinson Disease CBIGi044-A (LRRK2 N551K-R1398H-K1423K (protective haplotype)(CBIGi044-A), IPSC0058) Donor's gene variants: LRRK2, LRRK2 Donor diseases: Parkinson Disease CBIGi045-A (GBA L444P(CBIGi045-A), IPSC0077) Donor's gene variants: GBA, GBA Donor diseases: Parkinson Disease |
| Last update | 15th April 2022 |
| User feedback | |
Provider |
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| Generator | Fujian Medical University Union Hospital (FJMUUH) |
| Owner | Fujian Medical University Union Hospital (FJMUUH) |
| Distributors | |
| Derivation country | China |
External Databases |
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| BioSamples | SAMEA7211865 |
| Cellosaurus | CVCL_A1WA |
| Wikidata | Q105506873 |
General Information |
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| Publications |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | HAN |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Unknown
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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External Databases (Donor) |
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| BioSamples | SAMEA7211866 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | Controlled Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | the Ethics Committee of Fujian Medical University Union Hospital |
| Approval number | 2018-25 |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | Yes |
| Name of accrediting authority involved? | the Ethics Committee of Fujian Medical University Union Hospital |
| Approval number | 2018-25 |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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| Source cell type |
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Episomal |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
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| Files and images showing reprogramming vector expressed or silenced | |
| Vector map | |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Medium |
Essential 8™
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| NANOG |
Yes |
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| SOX2 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
Morphology pictures
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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