FJMUUHi001-A

General#

Cell Line

hPSCreg Name FJMUUHi001-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 28th July 2020
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Provider

Generator Fujian Medical University Union Hospital (FJMUUH)
Owner Fujian Medical University Union Hospital (FJMUUH)
Distributors
Derivation country China

External Databases

BioSamples SAMEA7211865
CLO CLO_0102556

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed

Donor Information#

General Donor Information

Sex male
Ethnicity HAN

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Parkinson's disease 7
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • autosomal recessive early-onset Parkinson's disease 7
  • autosomal recessive early-onset Parkinson disease 7
Genetic variants
PARK7 (target)
1p36.23
NC_000001.11:c.189dupA
NP_001116849.1: (p.Glu64Argfs)
Homozygous
The patient with the mutation has been reported in the article "DJ-1 in early-onset Parkinson disease:a case report and analysis "(DOI:10.3969/j.issn.1009-6574.2019.01.007).

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA7211866

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: Ye Qin-yong,deputy director of department of Neurology, Fujian Medical University Union Hospital, Fuzhou 350005, China. E-mail address: unionqyye@163.com.
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? Yes
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? No
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? the Ethics Committee of Fujian Medical University Union Hospital
Approval number 2018-25
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? the Ethics Committee of Fujian Medical University Union Hospital
Approval number 2018-25
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

Source cell type
Induced Pluripotent Stem Cell
Adult cells that have been genetically reprogrammed to an embryonic stem cell-like state by the expression of genes and factors important for maintaining the defining properties of embryonic stem cells.
Synonyms
  • iPSC
  • Induced Pluripotent Stem Cell

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
No
Methods used
PCR
Files and images showing reprogramming vector expressed or silenced
Vector map

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Medium Essential 8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
SOX2
Yes
POU5F1 (OCT-4)
Yes
Morphology pictures
Differentiation Potency
Intestinal Epithelium
Ont Id: NCIT_C49240
In vivo teratoma
Morphology
gut-like epithelium
Cartilage Element
Ont Id: UBERON_0007844
In vivo teratoma
Morphology
Neuroepithelial Stem Cell
Ont Id: CL_0002259
In vivo teratoma
Morphology

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)