BCHi001-A

General#

Cell Line

hPSCreg Name
BCHNi001-A
Alternative name(s)
BCHi001-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 27th July 2021
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Provider

Generator Beijing Children′s Hospital:Department of Nephrology (BCHN)
Owner Beijing Children′s Hospital:Department of Nephrology (BCHN)
Distributors
Derivation country China

External Databases

BioSamples SAMEA9465455
CLO CLO_0103449

General Information

* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Sex male
Ethnicity Chinese

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Bardet-Biedl Syndrome
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • BBS
  • Bardet-Biedl Syndrome
  • Laurence-Moon-Bardet-Biedl Syndrome
  • Laurence-Moon Syndrome
  • Laurence-Moon-Biedl Syndrome
show more synonyms
Disease associated phenotypes
  • Renal failure
  • Polydactyly
  • Fat
  • Myopia
  • Astigmatism
  • Intelligence deficiency
Family history None
Is the medical history available upon request? No
Is clinical information available? No

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA9465456

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: fuqian0224@126.com
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Yes
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? No
Does consent expressly prevent development of commercial products? No
Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? No
Does consent expressly permit storage of donated embryo/tissue for an unlimited time? No
Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? No
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
Does consent expressly permit collection of genetic information? Yes
Does consent expressly permit storage of genetic information? Yes
Does consent prevent dissemination of genetic information? No
Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? No
Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? Yes
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? Yes
Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? Yes
Does consent permit access to medical records of the donor? Yes
Please describe how access is provided: fuqian0224@126.con
Does consent permit access to any other source of information about the clinical treatment or health of the donor? Yes
Contact data, institution, or website: fuqian0224@126.con
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethics Committee of Beijing Children's Hospital Affiliated to Capital Medical University
Approval number 2021-E-047-Y
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? No
Do you have obligations to third parties in regard to the use of the cell line? No
Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? No
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? CytoTuneTM-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher Scientific)
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation#

General

Source cell type
Peripheral Blood Mononuclear Cell Sample
A sample of peripheral blood mononuclear cells.
Synonyms
  • Peripheral Blood Mononuclear Cell Specimen
  • PBMC Specimen
  • PBMC Sample
  • PBMC
  • Peripheral Blood Mononuclear Cell Sample
show more synonyms

Reprogramming method

Vector type Integrating
Vector Virus ()
Is the used vector excisable?
Yes
Absence of reprogramming vector(s)?
No
Reprogramming vectors silenced?
Yes
Methods used
RT-PCR

Vector free reprogramming

Other

Selection criteria for clones After the clone was formed, the single iPSC clone was observed and picked out, and the undifferentiated single clone was transferred to a new Matrigel (Biocoat)-coated cell plate. The nuclear expression of the pluripotency-related genes OCT4, NANOG and SOX2 was confirmed by immunofluorescence staining . The presence of pluripotency surface markers recognized by anti-SSEA4 antibodies in the cytoplasm was demonstrated by flow cytometric analysis.
Derived under xeno-free conditions
Yes
Derived under GMP?
Unknown
Available as clinical grade?
No

Culture Conditions#

Medium Essential 8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-4
Yes
NANOG
Yes
SOX2
Yes
POU5F1 (OCT-4)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Other
Marker Expressed
SOX17
Yes
Morphology
Mesoderm
Ont Id: UBERON_0000926
Other
Marker Expressed
SMA
Yes
Morphology
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
TUBLIN
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Passage number: 10
Karyotyping method: G-Banding

Other Genotyping (Cell Line)