Generation of an induced pluripotent stem cell line, FJMUUHi001-A, from a hereditary Parkinson's disease patient with homozygous mutation of c.189dupA in PARK7
Summary
PARK7 mutations are accountable for the inherited Parkinson's disease. An induced pluripotent stem cell (iPSC) line FJMUUHi001-A was generated by expressing five reprogramming factors, OCT3/4, SOX2, c-MYC, KLF4 and BCL-XL, in peripheral blood mononuclear cells from a 32-year old patient carrying a homozygous mutation of c.189dupA in PARK7. The iPSCs with a normal karyotype had the abilities to differentiate into three germ layers and expressed pluripotency markers without detectable residual plasmids. The cell line FJMUUHi001-A carrying the truncating protein PARK7 could be a useful tool to help comprehend the function of PARK7 in the iPSCs and differentiated cells from them. Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Chen ZT, Zhao ZH, Chen LN, Fan F, Cai GE, Weng HD, Wang YQ, Liao LM, Chen XC, Huang E, Ye QY |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2021 Mar;51:102175 |
| PubMed | 33485186 |
| DOI | 10.1016/j.scr.2021.102175 |