Generation of two induced pluripotent stem cell lines from catecholaminergic polymorphic ventricular tachycardia patients carrying RYR2 mutations
Summary
Catecholaminergic polymorphic ventricular tachycardia (CPVT) is a congenital arrhythmic syndrome caused by the RYR2 gene encoded ryanodine receptor. Mutations on RYR2 are commonly associated with ventricular tachycardia after adrenergic stimulation, leading to lethal arrhythmias and sudden cardiac death. We generated two human induced pluripotent stem cell (iPSC) lines from CPVT affected patients carrying single missense heterozygote RYR2 mutations, c.1082 G > A and c.100 A > C. Pluripotency and differentiation capability into derivatives of three germ layers were evaluated along with karyotype stability in the report. The generated patient-specific iPSC lines provide a reliable tool to investigate the CPVT phenotype and understand underlaying mechanisms. Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Kong X, Belbachir N, Zeng W, Yan CD, Navada S, Perez MV, Wu JC |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2023 Jun;69:103111 |
| PubMed | 37210947 |
| PubMed Central | PMC11059235 |
| DOI | 10.1016/j.scr.2023.103111 |