Generation of an induced pluripotent stem cell line, IBMS-iPSC-014-05, from a female autosomal dominant polycystic kidney disease patient carrying a common mutation of R803X in PKD2
Summary
Autosomal dominant polycystic kidney disease (ADPKD) is one of the most commonly inherited forms of polycystic kidney disease, and is characterized by the growth of numerous cysts in both kidneys. Here we generated an induced pluripotent stem cell (iPSC) line from the peripheral blood mononuclear cells (PBMCs) of a 63-year-old female ADPKD patient carrying an R803X mutation in the PKD2 gene using the Sendai-virus delivery system. Downstream characterization of these iPSCs showed that they possessed normal karyotyping, were free of genomic integration, retained the disease-causing PKD2 mutation, expressed pluripotency markers and could differentiate into three germ layers. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Ho MC, Huang CY, Lee JJ, Hsu SH, Cheng YC, Hsu YH, Hwang DY, Lu HE, Chen HC, Hsieh PCH |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2017 Dec;25:38-41 |
| PubMed | 29055226 |
| DOI | 10.1016/j.scr.2017.10.005 |