Generation of a human embryonic stem cell reporter line, TMEM119-EGFP, for the visualisation of in vitro differentiated human microglia
Summary
Transmembrane protein 119 (TMEM119) is a recently identified microglia marker that is not expressed by other immune cells. Using CRISPR/Cas9 technology, we introduced enhanced green fluorescence protein (EGFP), into the H9 WA-09 human embryonic stem cell line, directly before the TMEM119 stop codon. Sanger sequencing confirmed successful insertion of the EGFP sequence. The newly created cell line expressed a normal morphology and karyotype, several pluripotency markers, and the ability to differentiate into all three germ layers. H9-TMEM119-EGFP can be used to provide a deeper understanding of the role of TMEM119 in microglia by monitoring its expression under different experimental conditions. Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Denman CR, Tran HD, Shin MK, Ryu J, Kuhn B, Jo J |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2023 Dec;73:103264 |
| PubMed | 38029556 |
| DOI | 10.1016/j.scr.2023.103264 |