FHUSTCi001-A

General

Cell Line

hPSCreg Name
FHUSTCi001-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 16th March 2022
User feedback
Written by xin yu on 2021-12-27 14:37:03  
Highlights • An iPSC line from a Glomerulopathy with Fibronectin Deposits patient with a heterozygous c.5602G>A (p.V1868M) mutation in the FN1 gene. • Characterization of the GFND patient iPSC line and cultured it over the passage-10. • Confirmation of the pluripotency and cell line identity of this iPSC.

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Provider

Generator The First Affiliated Hospital of USTC,Division of life Science and Medicine,University of Science and Technology of China (FHUSTC)

External Databases

BioSamples SAMEA12056141
CLO CLO_0103643

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

External Databases (Donor)

BioSamples SAMEA12056140

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethics Committee of the First Affiliated Hospital of USTC,Division of life Science and Medicine,University of Science and Technology of China
Approval number 2021KY-219
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell line name peripheral blood monouclear cells
Derived from same source line (potentially other lot and donor, see below):
Source cell type
Autologous Peripheral Blood Mononuclear Cells
A preparation of autologous peripheral blood mononuclear cells (PBMCs).
Synonyms
  • Autologous Peripheral Blood Mononuclear Cell
  • Autologous PBMC
  • Autologous PBMCs
  • Autologous Peripheral Blood Mononuclear Cells
  • Therapeutic PBMCs
show more synonyms
Source cell origin
Blood
A liquid tissue; its major function is to transport oxygen throughout the body. It also supplies the tissues with nutrients, removes waste products, and contains various components of the immune system defending the body against infection. Several hormones also travel in the blood.
Synonyms
  • Reticuloendothelial System, Blood
  • Peripheral Blood
  • peripheral blood
  • blood
  • Blood
  • Whole Blood
  • BLOOD
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Episomal

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium mTeSR™ 1

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SOX2
Yes
NANOG
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Cartilage Element
Ont Id: UBERON_0007844
In vivo teratoma
Neuron
Ont Id: CL_0000540
In vivo teratoma

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes

Other Genotyping (Cell Line)