CMC-Fb-002
CMCi010-A
General#
Cell Line |
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hPSCreg Name | CMCi010-A |
Alternative name(s) |
CMC-Fb-002
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Last update | 30th November 2020 |
User feedback | |
Provider |
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Generator | Catholic University of Korea (CMC) |
Owner | Catholic University of Korea (CMC) |
External Databases |
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BioSamples | SAMEA7807674 |
CLO | CLO_0103129 |
Cellosaurus | CVCL_A4EV |
General Information |
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* Is the cell line readily obtainable for third parties? |
No |
Donor Information#
General Donor Information |
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Sex | female |
Ethnicity | Asian |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
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Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
Yes
46 XX
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External Databases (Donor) |
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BioSamples | SAMEA7807674 |
Ethics#
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
Was the consent voluntarily given? | Yes |
Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
Provide contact information of the holder of the original Donor Information Sheet: | cttailor@catholic.ac.kr |
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? | No |
If you do not hold the SIGNED Donor Consent Form, do you know who does? | Yes |
Contact information / weblink | cttailor@catholic.ac.kr |
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? | No |
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | Yes |
How may genetic information associated with the cell line be accessed? | No information |
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
Name of accrediting authority involved? | |
Approval number | |
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | Yes |
Name of accrediting authority involved? | |
Approval number | |
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation#
General |
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Source cell line name |
peripheral blood monouclear cells Derived from same source line (potentially other lot and donor, see below):
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Source cell type |
Derived Peripheral Blood Mononuclear CellMononuclear cells collected from peripheral blood.
Synonyms
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Reprogramming method |
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Vector type | None |
Vector free reprogramming |
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Type of used vector free reprogramming factor(s) |
None
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Other |
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Derived under xeno-free conditions |
Unknown |
Derived under GMP? |
Unknown |
Available as clinical grade? |
Unknown |
Culture Conditions#
Surface coating | Matrigel/Geltrex | ||||||
Feeder cells |
No |
||||||
Medium |
mTeSR™ 1
Supplements
|
||||||
Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
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Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
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Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
Score:
Marker | Present | Absent |
mCpG | ||
OCT4 |
Differentiation Potency
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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