CMCi006-A-1

CMC-Fb001(A4GALT-KO)

General

Cell Line

hPSCreg Name
CMCi006-A-1
Alternative name(s)
CMC-Fb001(A4GALT-KO)
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 7th September 2022
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Provider

Generator Catholic University of Korea (CMC)

External Databases

BioSamples SAMEA111327964
CLO CLO_0104049

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of

Donor Information

General Donor Information

Sex male
Ethnicity Asian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Fabry Disease
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Fabry Disease
  • Alpha-Galactosidase A Deficiency
  • Angiokeratoma Corporis Diffusum
  • Fabry's Disease
show more synonyms

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46,XY

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

All cell lines of this donor's relatives
Has mother:

External Databases (Donor)

BioSamples SAMEA8359738

Ethics

Also have a look at the ethics information for the parental line CMCi006-A .
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General

The source cell information can be found in the parental cell line CMCi006-A.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
Unknown

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
SSEA-4
Yes
TRA 1-81
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Morphology

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Passage number: 3

Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications
Fabry Disease
Synonyms
  • Fabry Disease
  • Alpha-Galactosidase A Deficiency
  • Angiokeratoma Corporis Diffusum
  • Fabry's Disease
show more synonyms
Genetic modifications
A4GALT (target)
Gene knock-out
22q11.2
CRISPR-associated (CRISPR/Cas) System