General

Cell Line
hPSCreg name	UKWNLi009-A-1
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	UKWNLi009-A-1
Alternative name(s)	IsoTK8, IsoFD-1
Cell line type	Human induced pluripotent stem cell (hiPSC)
Similar lines	No similar lines found.
Last update	21st August 2024
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Provider
Generator	Neurologische Klinik (UKWNL) Contact: Neurologische Klinik (UKWNL)
External Databases
BioSamples	SAMEA115942286
General Information
* Is the cell line readily obtainable for third parties?	No
Subclone of	UKWNLi009-A

Donor Information

General Donor Information
Sex	male
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Fabry Disease The donor is a carrier of a disease-associated mutation and affected. Synonyms Fabry Disease Alpha-Galactosidase A Deficiency Fabry's Disease
External Databases (Donor)
BioSamples	SAMEA115797737

Ethics

Also have a look at the ethics information for the parental line UKWNLi009-A .

For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

IDT

hIPSC Derivation

General
The source cell information can be found in the parental cell line UKWNLi009-A.
Reprogramming method
Vector type	None
Vector free reprogramming
Type of used vector free reprogramming factor(s)	mRNA
Other
Derived under xeno-free conditions	Unknown
Derived under GMP?	Unknown
Available as clinical grade?	Unknown

Culture Conditions

Medium

Other medium: Base medium: StemMACS™ iPS-Brew XF, human Main protein source: Serum concentration: %

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
NANOG	Yes
SSEA-4	Yes
TRA 1-60	Yes

Differentiation Potency

Endoderm

Endoderm
Ont Id: UBERON_0000925

In vitro directed differentiation

Mesoderm

Mesoderm
Ont Id: UBERON_0000926

In vitro directed differentiation

Ectoderm

Microbiology / Virus Screening
Mycoplasma	Negative

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46, XY
Other Genotyping (Cell Line)

Genetic Modification

Disease/phenotype related modifications	Fabry Disease Isogenic control to a Fabry Disease cell line Synonyms Fabry Disease Alpha-Galactosidase A Deficiency Fabry's Disease Genetic modifications GLA (target) Type of modification Isogenic modification
Genetic modifications not related to a disease