CMC-Fb-001

General

Cell Line

hPSCreg name CMCi006-A
Cite as:
CMCi006-A (RRID:CVCL_A4EQ)
Alternative name(s)
CMC-Fb-001
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
CMCi006-A-1
(CMC-Fb001(A4GALT-KO))
Donor diseases:
Fabry Disease
CMCi007-A
(CMC-Fb-004)
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Fabry Disease
INSAi002-A
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Fabry Disease
UKWNLi006-B
(GLA-D170N-iPSC-2, GLA-508G>A-2, FD1089/21)
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Fabry Disease
UKWNLi007-B
(GLA-515-G>A-2, FD1210/2, GLA-C172Y-iPSC-2)
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Fabry Disease
CTGUi001-A
(FD01, F01)
Donor diseases:
Fabry Disease
UKWNLi006-A
(GLA-D170N-iPSC-1, FD1089/20, GLA-508G>A-1)
Donor diseases:
Fabry Disease
UKWNLi007-A
(GLA-515G>A-1, GLA-C172Y-iPSC-1, FD1210/1)
Donor diseases:
Fabry Disease
CMCi010-A
(CMC-Fb-002)
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Fabry Disease
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Fabry disease
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NJDTHi001-A
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AHQUi001-A
(IPS35-2)
Donor's gene variants:
LPL
Donor diseases:
NON RARE IN EUROPE: Hyperlipoproteinemia type 4
SDQLCHi030-A
Donor's gene variants:
HPRT1
Donor diseases:
Lesch-Nyhan Syndrome
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PCIi005-A
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Last update 7th September 2022
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Provider

Generator Catholic University of Korea (CMC)
Owner Catholic University of Korea (CMC)
Derivation country South Korea

External Databases

BioSamples SAMEA7807666
Cellosaurus CVCL_A4EQ
Wikidata Q105506649

General Information

Publications
* Is the cell line readily obtainable for third parties?
No
Subclones

Donor Information

General Donor Information

Sex male
Ethnicity Asian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Fabry Disease
  • Alpha-Galactosidase A Deficiency
  • Angiokeratoma Corporis Diffusum
  • Fabry's Disease
show more synonyms

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46 XY
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

All cell lines of this donor's relatives
Has mother:

External Databases (Donor)

BioSamples SAMEA8359738

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. cttailor@catholic.ac.kr
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information cttailor@catholic.ac.kr
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Institutional Review Board of St. Vincent Hospital
Approval number VC17TISI0015
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Institutional Review Board of St. Vincent Hospital
Approval number VC17TISI0015
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • PBMC

Reprogramming method

Vector type None

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Medium mTeSR™ 1
Supplements
5X 100 ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Score:
Marker Present Absent
mCpG
OCT4
Morphology pictures
Self-renewal
Positive
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Morphology
Mesoderm
Ont Id: UBERON_0000926
Morphology
Ectoderm
Ont Id: UBERON_0000924
Morphology

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XY
Karyotyping method: G-Banding

Other Genotyping (Cell Line)