NJDTHi001-A

General#

Cell Line

hPSCreg Name NJDTHi001-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 21st August 2020
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Department of Cardio-Thoracic Surgery, Nanjing Drum Tower Hospital (NJDTH)
Owner Department of Cardio-Thoracic Surgery, Nanjing Drum Tower Hospital (NJDTH)
Derivation country China

External Databases

BioSamples SAMEA7346334

General Information

* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Sex male
Ethnicity Han

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Danon disease
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • ANTOPOL DISEASE
  • PSEUDOGLYCOGENOSIS II

External Databases (Donor)

BioSamples SAMEA7346333

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? Yes
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? No
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Ethics Committee of Nanjing Drum Tower Hospital
Approval number 2016-151-01
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

Source cell type
Peripheral Blood Mononuclear Cell
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • Peripheral Blood Mononuclear cells
  • PBMC
show more synonyms
Collected in 2020

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Yes
Available as clinical grade?
Yes

Culture Conditions#

Surface coating Matrigel/Geltrex
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
SSEA-4
Yes
POU5F1 (OCT-4)
Yes
Marker Present Absent
mCpG
OCT4 X
Report
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
PAX6
Yes
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
TBXT
Yes
Morphology
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
AFP
Yes
Morphology

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46 XY

Other Genotyping (Cell Line)