Generation of four patient-specific pluripotent induced stem cell lines from two Brazilian patients with amyotrophic lateral sclerosis and two healthy subjects


Induced pluripotent stem cell (iPSC) lines were generated from erythroblasts of two patients with amyotrophic lateral sclerosis (ALS) and two healthy individuals. One familial and one sporadic ALS patients were used, both with genetic alterations in VAPB gene. CytoTune™-iPS 2.0 Sendai Reprogramming Kit (containing the reprogramming factors OCT3/4, KLF4, SOX2 and cMYC) was used to generate the iPSC cell lines. The four iPSCs express pluripotency markers, have normal karyotype and differentiated spontaneously in the three germ layers. The expression of Sendai virus was lost in all iPSC lines after 15 passages. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Gubert F, Vasques JF, Cozendey TD, Domizi P, Toledo MF, Kasai-Brunswick TH, Loureiro MPS, Lima JMB, Gress CH, Cabello GMK, Cabello PH, Borgonovo T, Vaz IM, Silva R, Mendez-Otero R
Journal Stem cell research
Publication Date 2019 May;37:101448
PubMed 31077962
DOI 10.1016/j.scr.2019.101448

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