ND2-tdTom1
NHLBIi003-A-1
General
Donor Information
General Donor Information |
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| Sex | male |
| Ethnicity | unknown |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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External Databases (Donor) |
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| BioSamples | SAMEA6348718 |
Ethics
Also have a look at the ethics information for the parental line
NHLBIi003-A
.
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
NHLBIi003-A.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Episomal |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
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| O2 Concentration | 21 % |
| CO2 Concentration | 5 % |
| Medium |
Essential 8™
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| SSEA-4 |
Yes |
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| NANOG |
Yes |
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| TRA 1-60 |
Yes |
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| SOX2 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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Morphology pictures
Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46, XY
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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Genetic Modification
| Genetic modifications not related to a disease |
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