Generation of two tdTomato reporter induced pluripotent stem cell lines (NHLBIi003-A-1 and NHLBIi003-A-2) by AAVS1 safe harbor gene-editing
Summary
Human induced pluripotent stem cells (iPSCs) that express stable and robust fluorescent proteins have proven to be indispensable in basic and translational research. These reporter iPSC lines can greatly facilitate cell imaging, sorting, and tracking in vitro and in vivo studies. Here, we document two reporter human iPSC lines generated by gene-editing technologies that precisely integrated one-copy of a tdTomato transgene driven by strong CAG promoter into the AAVS1 human safe harbor locus. Published by Elsevier B.V.
| Authors | Patterson K, Linask KL, Beers J, Zou J |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2020 Jan;42:101673 |
| PubMed | 31869686 |
| PubMed Central | PMC7046126 |
| DOI | 10.1016/j.scr.2019.101673 |