Generation of two tdTomato reporter induced pluripotent stem cell lines (NHLBIi003-A-1 and NHLBIi003-A-2) by AAVS1 safe harbor gene-editing


Human induced pluripotent stem cells (iPSCs) that express stable and robust fluorescent proteins have proven to be indispensable in basic and translational research. These reporter iPSC lines can greatly facilitate cell imaging, sorting, and tracking in vitro and in vivo studies. Here, we document two reporter human iPSC lines generated by gene-editing technologies that precisely integrated one-copy of a tdTomato transgene driven by strong CAG promoter into the AAVS1 human safe harbor locus. Published by Elsevier B.V.

Authors Patterson K, Linask KL, Beers J, Zou J
Journal Stem cell research
Publication Date 2020 Jan;42:101673
PubMed 31869686
PubMed Central PMC7046126
DOI 10.1016/j.scr.2019.101673

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