FH 3.2.8T

General#

Cell Line

hPSCreg Name
ICGi037-A
Alternative name(s)
FH 3.2.8T
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 5th December 2021
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Provider

Generator Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG)
Owner Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (ICG)
Distributors
Derivation country Russia

External Databases

BioSamples SAMEA11421028

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Hyperlipoproteinemia Type IIa
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • FH
  • Hyperlipidemia Type IIa
  • Hyperlipoproteinemia Type IIa
  • Type IIa Hyperlipidemia
  • Familial Hypercholesterolemia
show more synonyms
Genetic variants
LDLR (target)
19p13.2
NM_000527.4:c.2141-966_2390-330del
NP_000518.1:p.Glu714_Ile796del
LDLR (target)
19p13.2
NM_000527.4:c.1327T>C
NP_000518.1:p.Trp443Arg
Disease associated phenotypes
  • familial hypercholesterolemia

External Databases (Donor)

BioSamples SAMEA11421030

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Federal State Budgetary Institution NATIONAL MEDICAL RESEARCH CENTER OF CARDIOLOGY Ministry of Health of the Russian Federation
Approval number 246
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

Source cell type
Peripheral Blood Mononuclear Cell
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • Peripheral Blood Mononuclear cells
  • PBMC
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Episomal

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Feeder cells
Yes
Passage method Enzymatically
TrypLE
CO2 Concentration 5 %
Medium Other medium:
Base medium: koDMEM
Main protein source: Knock-out serum replacement
Serum concentration: 15 %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60
Yes
SSEA-4
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
Morphology pictures
FH3-2-8T morphology.tif
FH3-2-8T morphology
Differentiation Potency
Endoderm
Ont Id: NCIT_C12706
In vitro spontaneous differentiation
Marker Expressed
FOXA2
Yes
KRT18
Yes
Morphology
FH 3-2-8T HNF3b.tif
FH 3-2-8T HNF3b (FOXA2)
FH 3-2-8T KRT18.tif
FH 3-2-8T KRT18
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
ACTA2
Yes
CD90
Yes
Morphology
FH 3-2-8T aSMA.tif
FH 3-2-8T aSMA (ACTA2)
FH 3-2-8T CD90.tif
FH 3-2-8T CD90
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
TUBB3
Yes
GFAP
Yes
Morphology
FH 3-2-8T TUBB3.tif
FH 3-2-8T TUBB3
FH 3-2-8T GFAP.tif
FH 3-2-8T GFAP

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Passage number: 10
Karyotyping method: G-Banding

Other Genotyping (Cell Line)