Generation of RYBP FLAG-HA knock-in human embryonic stem cell line through CRISPR/Cas9-mediated homologous recombination


RYBP, a critical component of polycomb repressive complex1 (PRC1), is required for the pluripotency and differentiation of mouse embryonic stem cells(mESCs). However, its function and mechanism to regulate human embryonic stem cells(hESCs) remain unknown. Here, to investigate the role of RYBP in hESCs, we generate an hESC line with FLAG-HA tag knock-in to RYBP locus through CRISPR/Cas9-mediated homologous recombination. hESC with RYBP_FLAG-HA knock-in maintains normal morphology and karyotype, while it maintains pluripotency to differentiate into three germ layers. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Zhang J, Zhou T, Shan Y, Pan G
Journal Stem cell research
Publication Date 2022 Jul;62:102803
PubMed 35537243
DOI 10.1016/j.scr.2022.102803

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