Process control and in silico modeling strategies for enabling high density culture of human pluripotent stem cells in stirred tank bioreactors


The routine therapeutic and industrial applications of human pluripotent stem cells (hPSCs) require their constant mass supply by robust, efficient, and economically viable bioprocesses. Our protocol describes the fully controlled expansion of hPSCs in stirred tank bioreactors (STBRs) enabling cell densities of 35 × 106 cells/mL while reducing culture medium consumption by 75%. This is achieved by in silico process modeling and computable upscaling. We provide a detailed blueprint for systematic process development of hPSCs and their progenies. For complete details on the use and execution of this protocol, please refer to Manstein et al. (2021). © 2021 The Author(s).

Authors Manstein F, Ullmann K, Triebert W, Zweigerdt R
Journal STAR protocols
Publication Date 2021 Dec 17;2(4):100988
PubMed 34917976
PubMed Central PMC8666714
DOI 10.1016/j.xpro.2021.100988

Research Projects

Cell Lines