hHSC_Iso4_ADCF_SeViPS2 (Phönix)

General

Cell Line

hPSCreg name MHHi001-A
Cite as:
MHHi001-A (RRID:CVCL_QX51)
Alternative name(s)
hHSC_Iso4_ADCF_SeViPS2 (Phönix)
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
MHHi001-A-7
(NRF2_A_Phönix-iPSC clone19)
MHHi001-A-13
(Phx_SRCAP_g3_1200_7)
MHHi001-A-6
(NRF2_A_Phönix-iPSC clone12)
MHHi001-A-12
(Phx_SRCAP_g3_1200_4)
Last update 29th March 2023
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Hannover Medical School (MHH)
Derivation country Germany

External Databases

BioSamples SAMEA4564584
Cellosaurus CVCL_QX51
Wikidata Q54905552

General Information

Publications
* Is the cell line readily obtainable for third parties?
No
Subclones

Donor Information

General Donor Information

Sex female
Age of donor (at collection) neonate
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history N/A
Is the medical history available upon request? no
Is clinical information available? no

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA4564585

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. Haidukiewicz, Marion, Medizinische Hochschule Hannover, Klinik für Frauenheilkunde und Geburtshilfe Haidukiewicz.Marion@mh-hannover.de
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? No
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent expressly permit collection of genetic information? Yes
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does consent permit access to medical records of the donor? No
Does consent permit access to any other source of information about the clinical treatment or health of the donor? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? MHH Ethikkommission
Approval number 1303-2012
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? MHH Ethikkommission
Approval number 1303-2012
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Synonyms
  • Hematopoietic stem cell
  • Blood-forming stem cell
Source cell origin
Any hematopoietic stem cell that is part of a umbilical cord blood.
Source cell type (free text) CD34 positive human cord blood hematopoietic stem cells (CD34+hCBHSCs)
Age of donor (at collection) neonate
Collected in 2014

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Yes
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Synthemax (Corning)
Feeder cells
No
Passage method Enzymatically
ReLeSR
Medium TeSR™ E8™

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
TRA 1-60
Yes
NANOG
Yes
SSEA-3
Yes
SSEA-4
Yes
POU5F1 (OCT-4)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
sarcomeric Actinin
Yes
cTroponin T
Yes
Desmin
Yes
Neuron
Ont Id: CL_0000540
In vitro spontaneous differentiation
Marker Expressed
TUBB3
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Passage number: 39

Other Genotyping (Cell Line)