hHSC_Iso4_ADCF_SeViPS2 (Phönix)

General#

Cell Line

hPSCreg Name MHHi001-A
Alternative name(s)
hHSC_Iso4_ADCF_SeViPS2 (Phönix)
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th December 2017
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Provider

Generator Hannover Medical School (MHH)
Derivation country Germany

External Databases

BioSamples SAMEA4564584
Cellosaurus CVCL_QX51

General Information

Publications
Is the cell line available in principle?
No

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA4564585

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
hematopoietic stem cell
A stem cell from which all cells of the lymphoid and myeloid lineages develop, including blood cells and cells of the immune system. Hematopoietic stem cells lack cell markers of effector cells (lin-negative). Lin-negative is defined by lacking one or more of the following cell surface markers: CD2, CD3 epsilon, CD4, CD5 ,CD8 alpha chain, CD11b, CD14, CD19, CD20, CD56, ly6G, ter119.
Synonyms
  • blood forming stem cell
  • HSC
  • hemopoietic stem cell
Source cell type (free text) CD34 positive human cord blood hematopoietic stem cells (CD34+hCBHSCs)

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60
Yes
NANOG
Yes
SSEA-3
Yes
SSEA-4
Yes
POU5F1 (OCT-4)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
sarcomeric Actinin
Yes
cTroponin T
Yes
Desmin
Yes
Neuron
Ont Id: CL_0000540
In vitro spontaneous differentiation
Marker Expressed
beta3 Tubulin
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)