hHSC_Iso4_ADCF_SeViPS2 (Phönix)
MHHi001-A
General#
Cell Line |
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| hPSCreg Name | MHHi001-A |
| Alternative name(s) |
hHSC_Iso4_ADCF_SeViPS2 (Phönix)
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Last update | 8th December 2017 |
| User feedback | |
Provider |
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| Generator | Hannover Medical School (MHH) |
| Derivation country | Germany |
External Databases |
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| BioSamples | SAMEA4564584 |
| Cellosaurus | CVCL_QX51 |
General Information |
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| Publications |
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| Is the cell line available in principle? |
No |
Donor Information#
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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External Databases (Donor) |
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| BioSamples | SAMEA4564585 |
Ethics#
We are currently revising this section.hIPSC Derivation#
General |
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| Source cell type |
hematopoietic stem cellA stem cell from which all cells of the lymphoid and myeloid lineages develop, including blood cells and cells of the immune system. Hematopoietic stem cells lack cell markers of effector cells (lin-negative). Lin-negative is defined by lacking one or more of the following cell surface markers: CD2, CD3 epsilon, CD4, CD5 ,CD8 alpha chain, CD11b, CD14, CD19, CD20, CD56, ly6G, ter119.
Synonyms
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| Source cell type (free text) | CD34 positive human cord blood hematopoietic stem cells (CD34+hCBHSCs) |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unkown |
| Derived under GMP? |
Unkown |
| Available as clinical grade? |
Unkown |
Culture Conditions#
| Medium |
mTeSR™ 1
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Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| TRA 1-60 |
Yes |
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| NANOG |
Yes |
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| SSEA-3 |
Yes |
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| SSEA-4 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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Differentiation Potency
In vitro spontaneous differentiation
In vitro spontaneous differentiation
| Marker | Expressed |
| sarcomeric Actinin |
Yes |
| cTroponin T |
Yes |
| Desmin |
Yes |
Genotyping#
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XX
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Other Genotyping (Cell Line) |
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