Generation of human induced pluripotent stem cell lines encoding for genetically encoded calcium indicators RCaMP1h and GCaMP6f


Calcium plays a key role in cardiomyocytes (CMs) for the translation of the electrical impulse of an action potential into contraction forces. A rapid, not-invasive fluorescence imaging technology allows for the monitoring of calcium transients in human induced pluripotent stem cell derived-cardiomyocytes (hiPSC-CMs) to investigate the cardiac electrophysiology in vitro and after cell transplantation in vivo. The genetically encoded calcium indicators (GECIs) GCaMP6f or RCaMP1h were successfully transfected in the previously established hiPSC line MHHi001-A, together with a cardiac specific antibiotic selection cassette facilitating the monitoring of the calcium handling in highly pure populations of hiPSC-CMs. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Ricci Signorini ME, Szepes M, Melchert A, Bakar M, Merkert S, Haase A, Göhring G, Martin U, Gruh I
Journal Stem cell research
Publication Date 2022 Apr;60:102697
PubMed 35152180
DOI 10.1016/j.scr.2022.102697

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