cyclinB1-eGFP hiPSCs
The cell line is not validated yet.
MHHi001-A-20
General
Cell Line |
|
| hPSCreg name | MHHi001-A-20 |
| Cite as: | MHHi001-A-20 |
| Alternative name(s) |
cyclinB1-eGFP hiPSCs
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 3rd February 2025 |
| User feedback | |
Provider |
|
| Generator |
Hannover Medical School (MHH)
Contact:
Hannover Medical School (MHH) |
| Owner | Hannover Medical School (MHH) |
| Distributors | |
| Derivation country | Germany |
External Databases |
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| BioSamples | SAMEA117736335 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Cell line can only be used in: we are happy to provide the cell line using an official Material Transfer Agreement for research use only.
Research use: allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Family history | N/A |
| Is the medical history available upon request? | no |
| Is clinical information available? | no |
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
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| BioSamples | SAMEA4564585 |
Ethics
Also have a look at the ethics information for the parental line
MHHi001-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
MHHi001-A.
|
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Reprogramming method |
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| Vector type | None |
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
None
|
Other |
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| Derived under xeno-free conditions |
Yes |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| O2 Concentration | 37 % |
| CO2 Concentration | 5 % |
| Medium |
Essential 8™
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Characterisation
Analysis of Undifferentiated Cells
| Marker | Present | Absent |
| mCpG | ||
| OCT4 | X |
Self-renewal
Unknown
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Differentiation Potency
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |
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Genetic Modification
| Genetic modifications not related to a disease |
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