Generation of two human NRF2 knockout iPSC clones using CRISPR/Cas9 editing


The nuclear factor erythroid 2-related factor 2 (NFE2L2, known as NRF2) regulates the expression of antioxidative and anti-inflammatory proteins. In order to investigate its impact during viral infections and testing of antiviral compounds, we applied CRISPR/Cas9 editing to eliminate NRF2 in the human iPS cell line MHHi001-A and generated two NRF2 knockout iPSC clones MHHi001-A-6 and MHHi001-A-7. After differentiation into epithelia or endothelial cells, these cells are useful tools to examine the antiviral effects of activators of the NRF2 signaling pathway. Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Merkert S, Haase A, Dahlmann J, Göhring G, Waqas FH, Pessler F, Martin U, Olmer R
Journal Stem cell research
Publication Date 2023 Jun;69:103090
PubMed 37104932
DOI 10.1016/j.scr.2023.103090

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