Generation of two human NRF2 knockout iPSC clones using CRISPR/Cas9 editing
The nuclear factor erythroid 2-related factor 2 (NFE2L2, known as NRF2) regulates the expression of antioxidative and anti-inflammatory proteins. In order to investigate its impact during viral infections and testing of antiviral compounds, we applied CRISPR/Cas9 editing to eliminate NRF2 in the human iPS cell line MHHi001-A and generated two NRF2 knockout iPSC clones MHHi001-A-6 and MHHi001-A-7. After differentiation into epithelia or endothelial cells, these cells are useful tools to examine the antiviral effects of activators of the NRF2 signaling pathway. Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.
|Authors||Merkert S, Haase A, Dahlmann J, Göhring G, Waqas FH, Pessler F, Martin U, Olmer R|
|Journal||Stem cell research|
|Publication Date||2023 Jun;69:103090|