Suspension culture improves iPSC expansion and pluripotency phenotype
Summary
This study demonstrates nearly 100-fold iPSC expansion over 5-days using our 3D suspension culture protocol in Vertical-Wheel® bioreactors, the largest cell growth reported to date. 3D expanded cells showed enhanced in vitro and in vivo pluripotency phenotype that may support more efficient scale-up strategies and safer clinical implementation. © 2023. The Author(s).
| Authors | Cuesta-Gomez N, Verhoeff K, Dadheech N, Dang T, Jasra IT, de Leon MB, Pawlick R, Marfil-Garza B, Anwar P, Razavy H, Zapata-Morin PA, Jickling G, Thiesen A, O'Gorman D, Kallos MS, Shapiro AMJ |
|---|---|
| Journal | Stem cell research & therapy |
| Publication Date | 2023 Jun 6;14(1):154 |
| PubMed | 37280707 |
| PubMed Central | PMC10245469 |
| DOI | 10.1186/s13287-023-03382-9 |