Generation of two TMEM16A knockout iPSC clones each from a healthy human iPSC line, from a Cystic Fibrosis patient specific line with p.Phe508del mutation and from the gene corrected iPSC line


The Transmembrane member 16A (TMEM16A), also known as anoctamin-1 (ANO1), is a calcium-activated chloride channel present in the airway epithelium. It is known to be involved in the apical chloride secretion indicating that TMEM16A could be addressed for the treatment of chloride secretion defects like in Cystic- Fibrosis (CF). In this paper we generated knockout cell lines using CRISPR/Cas9-mediated ablation in a healthy human iPSC line (MHHi001-A), in a CF patient iPSC line (MHHi002-A) and in its corrected counterpart (MHHi002-A-1). These lines can be used for gaining information about the role of TMEM16A for mucus secretion and/or production and evaluating its therapeutic potential. Copyright © 2022 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Jaboreck MC, Lühmann JL, Mielenz M, Stanke F, Göhring G, Martin U, Olmer R, Merkert S
Journal Stem cell research
Publication Date 2022 Oct;64:102918
PubMed 36162332
DOI 10.1016/j.scr.2022.102918

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