CF(1)iPS5

General#

Cell Line

hPSCreg Name MHHi002-A
Alternative name(s)
CF(1)iPS5
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th December 2017
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Provider

Generator Hannover Medical School (MHH)

External Databases

BioSamples SAMEA103885560
Cellosaurus CVCL_QX52

General Information

Publications
Is the cell line available in principle?
No
Subclones

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Cystic fibrosis
Cystic Fibrosis F508del homozygous
Synonyms
  • Mucoviscidosis
  • CF

External Databases (Donor)

BioSamples SAMEA7418668

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
endothelial cell
An endothelial cell comprises the outermost layer or lining of anatomical structures and can be squamous or cuboidal. In mammals, endothelial cell has vimentin filaments and is derived from the mesoderm.
Synonyms
  • endotheliocyte
Source cell type (free text) endothelia colony forming cell from peripheral blood

Reprogramming method

Vector type Integrating
Vector Virus (Lentivirus)
Is the used vector excisable?
Yes
Absence of reprogramming vector(s)?
No
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Medium Other medium:
Base medium: Knockout DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
SOX2
Yes
NANOG
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
SOX17
Yes
Cardiac Muscle Cell
Ont Id: CL_0000746
In vitro spontaneous differentiation
Marker Expressed
cTroponin T
Yes
Neuron
Ont Id: CL_0000540
In vitro spontaneous differentiation
Marker Expressed
beta3 Tubulin
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46[XY]

Other Genotyping (Cell Line)