CF(1)iPS5
MHHi002-A
General#
Cell Line |
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| hPSCreg Name | MHHi002-A |
| Alternative name(s) |
CF(1)iPS5
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Last update | 8th December 2017 |
| User feedback | |
Provider |
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| Generator | Hannover Medical School (MHH) |
External Databases |
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| BioSamples | SAMEA103885560 |
| Cellosaurus | CVCL_QX52 |
General Information |
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| Publications | |
| Is the cell line available in principle? |
No |
| Subclones | |
Donor Information#
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
|
External Databases (Donor) |
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| BioSamples | SAMEA7418668 |
Ethics#
We are currently revising this section.hIPSC Derivation#
General |
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| Source cell type |
endothelial cellAn endothelial cell comprises the outermost layer or lining of anatomical structures and can be squamous or cuboidal. In mammals, endothelial cell has vimentin filaments and is derived from the mesoderm.
Synonyms
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| Source cell type (free text) | endothelia colony forming cell from peripheral blood |
Reprogramming method |
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| Vector type | Integrating |
| Vector | Virus (Lentivirus) |
| Is the used vector excisable? |
Yes |
| Absence of reprogramming vector(s)? |
No |
| Reprogramming vectors silenced? | |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unkown |
| Derived under GMP? |
Unkown |
| Available as clinical grade? |
Unkown |
Culture Conditions#
| Medium |
Other medium:
Base medium: Knockout DMEM
Main protein source: Knock-out serum replacement Serum concentration: 20 % |
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
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| TRA 1-60 |
Yes |
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| SOX2 |
Yes |
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| NANOG |
Yes |
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Genotyping#
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46[XY]
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Other Genotyping (Cell Line) |
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