KCL-003-CF-1

General#

Cell Line

hPSCreg Name KCLe003-A
Alternative name(s)
KCL-003-CF-1
Cell line type Human embryonic stem cell (hESC)
Last update 27th October 2017
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Provider

Generator King's College London (KCL)
Derivation country United Kingdom

External Databases

Cellosaurus CVCL_A239

General Information

Publications
Is the cell line available in principle?
No

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Cystic fibrosis
The donor is affected.
Synonyms
  • Mucoviscidosis
  • CF

Ethics#

We are currently revising this section.

hESC Derivation#

Embryo stage Blastula with ICM and Trophoblast
PGD Embryo?
Yes
Expansion status 6
Stephenson 2007
ZP removal technique Enzymatic
Cell isolation Immunosurgery
Cell seeding Isolated ICM

Culture Conditions#

Feeder cells mouse embryonic fibroblast cell
Cellfinder Ont Id: EFO_0004040
Passage method Mechanically
O2 Concentration 21 %
CO2 Concentration 6 %
Medium Other medium:
Base medium: High glucose DMEM
Main protein source: Fetal Calf Serum
Serum concentration: 20 %
Supplements
Bovine Serum Albumin(BSA)

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
DNMT3B
Yes
FGF4
Yes
GABRB3
Yes
GDF3
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
ZFP42 (REX-1)
Yes
SOX2
Yes
TDGF1
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY

Other Genotyping (Cell Line)