IGGi002-A
General
Cell Line |
|
| hPSCreg Name | IGGi002-A |
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Last update | 23rd August 2023 |
| User feedback | |
Provider |
|
| Generator | IRCCS Istituto Giannina Gaslini (IGG) |
| Derivation country | Italy |
External Databases |
|
| BioSamples | SAMEA113585496 |
General Information |
|
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: allowed
|
Donor Information
General Donor Information |
|
| Sex | female |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
|
| Diseases | A disease was diagnosed.
|
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
Yes
46 XX
Karyotyping method:
KaryoStat+ with Cell ID Analysis (Thermo Fisher Scientific, A52986)
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
| BioSamples | SAMEA113585589 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Provide contact information of the holder of the original Donor Information Sheet: | 'fabiana.ciciriello@opbg.net' |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | pseudonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | No |
| Does consent expressly prevent the derivation of pluripotent stem cells? | No |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| How may genetic information associated with the cell line be accessed? | Open Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | Comitato Etico |
| Approval number | 2855 / 2022 |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
|
| Source cell line name | human nasal epithelial cells |
| Source cell type |
Nasal epithelium |
| Source cell origin |
nasal cavity mucosaThe mucous membrane that lines the nasal cavity[MP].
|
| Source cell type (free text) | mucous membranes of nasal turbinate |
| Passage number reprogrammed | 4 |
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
No |
| Methods used |
RT-PCR
|
| Notes on reprogramming vector detection | Clone #1 |
Vector free reprogramming |
|
Other |
|
| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | Vitronectin |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| CO2 Concentration | 5 % |
| Medium |
Essential 8™
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| SSEA-4 |
Yes |
|||||
| TRA 1-60 |
Yes |
|||||
| SOX2 |
Yes |
|||||
| POU5F1 (OCT-4) |
Yes |
Differentiation Potency
In vitro directed differentiation
| Marker | Expressed |
| forkhead box A2 |
Yes |
| SRY-box transcription factor 17 |
Yes |
In vitro directed differentiation
| Marker | Expressed |
| TBX6 – T-box transcription factor 6 |
Yes |
| TBXT T-box transcription factor T |
Yes |
In vitro directed differentiation
| Marker | Expressed |
| TUBB3 – tubulin beta 3 class III |
Yes |
| NES – nestin |
Yes |
| PAX6 – paired box 6 |
Yes |
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46 XX
Passage number: 10
Karyotyping method:
KaryoStat+ with Cell ID Analysis (Thermo Fisher Scientific, A52986)
|
Other Genotyping (Cell Line) |
|
Login to share your feedback, experiences or results with the research community.