General

Cell Line

hPSCreg name IGGi002-A
Cite as:
IGGi002-A (RRID:CVCL_D0FF)
Cell line type Human induced pluripotent stem cell (hiPSC)
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Last update 23rd August 2023
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Provider

Generator IRCCS Istituto Giannina Gaslini (IGG)
Derivation country Italy

External Databases

BioSamples SAMEA113585496
Cellosaurus CVCL_D0FF
Wikidata Q123032667

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: allowed

Donor Information

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
G542X/G542X
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Cystic Fibrosis
  • cystic fibrosis

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46 XX
Karyotyping method: KaryoStat+ with Cell ID Analysis (Thermo Fisher Scientific, A52986)

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA113585589

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. 'fabiana.ciciriello@opbg.net'
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Comitato Etico
Approval number 2855 / 2022
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell line name human nasal epithelial cells
Source cell type
Source cell origin
The mucous membrane that lines the nasal cavity[MP].
Synonyms
  • mucosa of nose
  • mucous membrane of nose
  • tunica mucosa nasalis
  • tunica mucosa nasi
  • nasal mucosa
show more synonyms
Source cell type (free text) mucous membranes of nasal turbinate
Passage number reprogrammed 4

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
RT-PCR
Notes on reprogramming vector detection Clone #1

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SSEA-4
Yes
TRA 1-60
Yes
SOX2
Yes
POU5F1 (OCT-4)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46 XX
Passage number: 10
Karyotyping method: KaryoStat+ with Cell ID Analysis (Thermo Fisher Scientific, A52986)

Other Genotyping (Cell Line)