CF(15)iPS10

General#

Cell Line

hPSCreg Name MHHi003-A
Alternative name(s)
CF(15)iPS10
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th December 2017
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Hannover Medical School (MHH)
Owner Leibniz Forschungslaboratorien für Biotechnologie und künstliche Organe (LEBAO)

External Databases

BioSamples SAMEA103885561
Cellosaurus CVCL_VE58

General Information

Is the cell line available in principle?
No

Donor Information#

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Cystic fibrosis
Cystic fibrosis, F508del homozygous
Synonyms
  • Mucoviscidosis
  • CF

External Databases (Donor)

BioSamples SAMEA103885562

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
mononuclear cell
A leukocyte with a single non-segmented nucleus in the mature form.
Synonyms
  • mononuclear leukocyte
Source cell type (free text) CD34 positive cells from peripheral blood

Reprogramming method

Vector type Integrating
Vector Virus (Lentivirus)
Is the used vector excisable?
Unkown
Absence of reprogramming vector(s)?
Unkown
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
Unkown
Derived under GMP?
Unkown
Available as clinical grade?
Unkown

Culture Conditions#

Medium Other medium:
Base medium: DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
NANOG
Yes
SOX2
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
Desmin
Yes
Neuron
Ont Id: CL_0000540
In vitro spontaneous differentiation
Marker Expressed
beta3 Tubulin
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY [15]

Other Genotyping (Cell Line)